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Literature summary for 3.1.6.8 extracted from
- Purification and properties of arylsulfatase. A from human urine (1975), J. Biol. Chem., 250, 2495-2501.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| taurodeoxycholate | enzyme cleaves cerebroside sulfates only in the presence of either specific detergent, e.g. taurodeoxycholate or human activator protein | Homo sapiens |  |
General Stability
| General Stability | Organism |
|---|---|
| 4°C, addition of dithiothreitol, glutathione, or EDTA reduce storage stability | Homo sapiens |
| bovine serum albumin stabilizes | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
cerebroside 3-sulfate | - |
Homo sapiens | |
| 6.7 | - |
4-nitrocatechol sulfate | - |
Homo sapiens | |
| 8 | - |
4-Methylumbelliferyl sulfate | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | - |
Homo sapiens | 5764 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | stimulates | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
SDS-PAGE | Homo sapiens |
| 100000 | - |
sedimentation equilibrium ultracentrifugation | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cerebroside 3-sulfate + H2O | Homo sapiens | - |
cerebroside + sulfate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| urine | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, protein: 1 mg/ml, stable for months | Homo sapiens |
| 4°C, pH 7.5, protein: 0.002 mg/ml, bovine serum albumin, insulin or ribonuclease, 15 days, no loss of activity | Homo sapiens |
| 4°C, pH 7.5, protein: 0.002 mg/ml, bovine serum albumin, insulin or ribonuclease, 8 months, 30% loss of activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cerebroside 3-sulfate + H2O | - |
Homo sapiens | cerebroside + sulfate | - |
? | |
| methylumbelliferyl sulfate + H2O | - |
Homo sapiens | methylumbelliferone + sulfate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * ca. 55000, SDS-PAGE | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.817 | - |
4-Methylumbelliferyl sulfate | - |
Homo sapiens | |
| 1.45 | - |
cerebroside 3-sulfate | - |
Homo sapiens | |
| 16.8 | - |
4-nitrocatechol sulfate | - |
Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
cerebroside sulfate | Homo sapiens |
| 5.2 | - |
4-methylumbelliferyl sulfate | Homo sapiens |
| 5.3 | - |
4-nitrocatechol sulfate | Homo sapiens |
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