Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sinorhizobium meliloti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
choline | competitive with respect to choline sulfate | Sinorhizobium meliloti | |
Choline sulfate | substrate inhibition | Sinorhizobium meliloti | |
additional information | not inhibitory: ammonium sulfate up to 70 mM | Sinorhizobium meliloti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.1 | - |
Choline sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
11.8 | - |
4-Methylumbelliferyl sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
75.8 | - |
4-nitrophenyl sulfate | pH 7.5, 25°C | Sinorhizobium meliloti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
118000 | - |
MALDI-TOF | Sinorhizobium meliloti |
123000 | - |
gel filtration | Sinorhizobium meliloti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | O69787 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | absence of formylglycine posttranslational modification in active-site cysteine54 | Sinorhizobium meliloti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl sulfate + H2O | - |
Sinorhizobium meliloti | methylumbelliferone + sulfate | - |
? | |
4-nitrophenyl sulfate + H2O | - |
Sinorhizobium meliloti | 4-nitrophenol + sulfate | - |
? | |
choline sulfate + H2O | - |
Sinorhizobium meliloti | choline + sulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 63000, SDS-PAGE, recombinant protein including His-tag, 2 * 59000, MALDI-TOF | Sinorhizobium meliloti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
4-nitrophenyl sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
0.043 | - |
4-Methylumbelliferyl sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
0.27 | - |
Choline sulfate | pH 7.5, 25°C | Sinorhizobium meliloti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Sinorhizobium meliloti |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
11.4 | - |
Choline sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
57.8 | - |
choline | pH 7.5, 25°C | Sinorhizobium meliloti |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00046 | - |
4-nitrophenyl sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
0.0036 | - |
4-Methylumbelliferyl sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
0.0243 | - |
Choline sulfate | pH 7.5, 25°C | Sinorhizobium meliloti |