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Literature summary for 3.1.6.2 extracted from

  • Uduwela, D.; Pabis, A.; Stevenson, B.; Kamerlin, S.; McLeod, M.
    Enhancing the steroid sulfatase activity of the arylsulfatase from Pseudomonas aeruginosa (2018), ACS Catal., 8, 8902-8914 .
No PubMed abstract available
Search for more literature for 3.1.6.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
I156L/K158E/L325F 140fold increase in catalyticefficiency, 3.5 degrees increase in melting temperature at pH 9.0 Pseudomonas aeruginosa
I156V/L157V/K158I/K330V 140fold increase in catalyticefficiency Pseudomonas aeruginosa
K330V catalytic efficiency similar to wild-type Pseudomonas aeruginosa
R155P 2.5fold increase in catalyticefficiency Pseudomonas aeruginosa
R155P/I156V/L325F/K330V 30fold increase in catalyticefficiency, 3.5 degrees increase in melting temperature at pH 9.0 Pseudomonas aeruginosa
R155P/K330V 5fold increase in catalyticefficiency Pseudomonas aeruginosa
R155P/L157V/K158I/K330V 150fold increase in catalyticefficiency Pseudomonas aeruginosa
R155T 2fold increase in catalyticefficiency Pseudomonas aeruginosa
R155TK330V 2fold increase in catalyticefficiency Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
 testosterone 3-sulfate mutant R155P/L157V/K158I/K330V, pH 9.0, 37°C Pseudomonas aeruginosa
0.0096
-
 testosterone 3-sulfate mutant I156V/L157V/K158I/K330V, pH 9.0, 37°C Pseudomonas aeruginosa
0.017
-
 testosterone 3-sulfate mutant I156L/K158E/L325F, pH 9.0, 37°C Pseudomonas aeruginosa
0.022
-
 testosterone 3-sulfate mutant R155P/K330V, pH 9.0, 37°C Pseudomonas aeruginosa
0.035
-
 testosterone 3-sulfate wild-type, pH 9.0, 37°C Pseudomonas aeruginosa
0.036
-
 testosterone 3-sulfate mutant R155P, pH 9.0, 37°C Pseudomonas aeruginosa
0.037
-
 testosterone 3-sulfate mutant R155T, pH 9.0, 37°C Pseudomonas aeruginosa
0.038
-
 testosterone 3-sulfate mutant R155P/I156V/L325F/K330V, pH 9.0, 37°C Pseudomonas aeruginosa
0.053
-
 testosterone 3-sulfate mutant K330V, pH 9.0, 37°C Pseudomonas aeruginosa
0.088
-
 testosterone 3-sulfate mutant R155T/K330V, pH 9.0, 37°C Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa P51691
-
-
Pseudomonas aeruginosa DSM 22644 P51691
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
testosterone 3-sulfate + H2O
-
 Pseudomonas aeruginosa testosterone + sulfate
-
 ?
testosterone 3-sulfate + H2O
-
 Pseudomonas aeruginosa DSM 22644 testosterone + sulfate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
51.2
-
 wild-type, melting temperature, pH 7.5 Pseudomonas aeruginosa
56
-
 wild-type, melting temperature, pH 9.0 Pseudomonas aeruginosa