Literature summary for 3.1.6.2 extracted from

Norkowska, M.; Gniot-Szulzycka, J.
Sterolsulphate sulphohydrolase from human placenta microsomes--30 kDa molecular weight form of cholesterol sulphate sulphohydrolase (2002), J. Steroid Biochem. Mol. Biol., 81, 263-271.

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Activating Compound

Activating Compound	Comment	Organism	Structure
ascorbic acid	activates	Homo sapiens
Digitonin	activates	Homo sapiens
estrone	slightly activates	Homo sapiens
glutathione	slightly activates	Homo sapiens
additional information	not activated by 2-mercaptoethanol, cysteine or dithiothreitol	Homo sapiens
p-chloromercuribenzoate	activates	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
AgNO3	5 mM, 65% inhibition	Homo sapiens
cholesterol	1 mM, 57% inhibition	Homo sapiens
cysteine	10 mM, 15% inhibition	Homo sapiens
KH2PO4	5 mM, 25% inhibition	Homo sapiens
additional information	not inhibited by estrone, dehydroepiandrosterone, alkaline phosphatase treatment and phosphohydrolase inhibitors	Homo sapiens
Na2SO3	5 mM, 43% inhibition	Homo sapiens
Na2SO4	5 mM, 18% inhibition	Homo sapiens
pregnenolone	1 mM, 10% inhibition	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0067	-	Cholesterol sulfate	pH 6.4, 37°C	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	placental, at least two distinct forms of CHS-ase	Homo sapiens	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	x * 30000, SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cholesterol sulfate + H2O	Homo sapiens	cholesterol sulfate metabolism, steroid sulfohydrolase pathway	cholesterol + sulfate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
solubilized with 0.5% Triton X-100, two fractions with cholesterol sulfate sulfohydrolase activity are separated by DEAE-cellulose chromatography, purification of the 30 kDa enzyme	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
placenta	microsomes, at least two distinct forms of CHS-ase	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.385	-	pH 6.4, 37°C	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cholesterol sulfate + H2O	cholesterol sulfate metabolism, steroid sulfohydrolase pathway	Homo sapiens	cholesterol + sulfate	-	?
cholesterol sulfate + H2O	highly specific for cholesterol sulfate	Homo sapiens	cholesterol + sulfate	-	?
dehydroepiandrosterone sulfate + H2O	1.5% of the activity with cholesterol sulfate	Homo sapiens	dehydroepiandrosterone + sulfate	-	?
estrone sulfate + H2O	0.6% of the activity with cholesterol sulfate	Homo sapiens	estrone + sulfate	-	?
pregnenolone sulfate + H2O	8.7% of the activity with cholesterol sulfate	Homo sapiens	pregnenolone + sulfate	-	?

Subunits

Subunits	Comment	Organism
?	x * 30000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
cholesterol sulfate sulfohydrolase	-	Homo sapiens
CHS-ase	-	Homo sapiens
sterolsulfate sulfohydrolase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	5 min, 40% loss of activity	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.4	-	-	Homo sapiens

pI Value

Organism	Comment	pI Value Maximum	pI Value
Homo sapiens	isoelectric focussing, pH-gradient 2-10	-	7.1

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Release 2023.1 (January 2023)