General Stability | Organism |
---|---|
lyophilization, deactivation | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | slight | Rattus norvegicus | |
AgNO3 | slight | Rattus norvegicus | |
CaCl2 | slight | Rattus norvegicus | |
EDTA | 10% inhibition at 1 mM | Rattus norvegicus | |
KBr | slight | Rattus norvegicus | |
KCl | slight | Rattus norvegicus | |
KCN | slight | Rattus norvegicus | |
LiCl | slight | Rattus norvegicus | |
Metabisulfite | - |
Rattus norvegicus | |
MgCl2 | slight | Rattus norvegicus | |
Na-metaborate | - |
Rattus norvegicus | |
Na-tetraborate | - |
Rattus norvegicus | |
Na2SO3 | slight | Rattus norvegicus | |
Na2SO4 | slight | Rattus norvegicus | |
NaCl | slight | Rattus norvegicus | |
NaF | slight | Rattus norvegicus | |
NaHSO3 | slight | Rattus norvegicus | |
SDS | - |
Rattus norvegicus | |
sodium arsenite | slight | Rattus norvegicus | |
sodium deoxycholate | - |
Rattus norvegicus | |
sodium taurocholate | - |
Rattus norvegicus | |
Steroids | overview, e.g. estrone, estradiol, C21 steroids, C19 steroids | Rattus norvegicus | |
Sulfate esters | overview, e.g. p-nitrophenyl sulfate | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | membrane-bound | Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
gel filtration | Rattus norvegicus |
130000 | - |
estrone sulfatase is identical to arylsulfatase C but not to sulfatases which hydrolyse dehydroepiabdrosterone, testosterone, pregnenolone, and cholesterol | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
steroid sulfates + H2O | Rattus norvegicus | also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site | steroid + sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
4oC, pH 7.4, imidazol buffer, several months, small loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol 3-sulfate + H2O | - |
Rattus norvegicus | cholesterol + sulfate | - |
? | |
dehydroandrosterone 3-sulfate + H2O | - |
Rattus norvegicus | dehydroandrosterone + sulfate | - |
? | |
dehydroepiandrosterone 3-sulfate + H2O | - |
Rattus norvegicus | dehydroepiandrosterone + sulfate | - |
? | |
estrone sulfate + H2O | - |
Rattus norvegicus | estrone + sulfate | - |
? | |
additional information | - |
Rattus norvegicus | ? | - |
? | |
p-nitrophenyl-sulfate + H2O | arylsulfatase activity, EC 3.1.6.1, and various steroid sulfatase activities (EC 3.1.6.1) are functions of the same protein | Rattus norvegicus | p-nitrophenol + sulfate | - |
? | |
pregnenolone 3-sulfate + H2O | - |
Rattus norvegicus | pregnenolone + sulfate | - |
? | |
steroid sulfates + H2O | also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site | Rattus norvegicus | steroid + sulfate | - |
? | |
testosterone 3-sulfate + H2O | - |
Rattus norvegicus | testosterone + sulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | arylsulfatase activity, E.C. 3.1.6.1, and various steroid sulfatase activities, E.C. 3.1.6.1, are functions of the same protein | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
10 min, 50% loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
dehydroepiandrosterone 3-sulfate | Rattus norvegicus |
8 | - |
esterone 3-sulfate | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | pH 5: about 25% of activity maximum, pH 8.5: about 15% of activity maximum, dehydroepiandrosterone 3-sulfate | Rattus norvegicus |