Any feedback?
Literature summary for 3.1.6.19 extracted from
- Structure and mechanism of an inverting alkylsulfatase from Pseudomonas sp. DSM6611 specific for secondary alkyl sulfates (2012), FEBS J., 279, 4374-4384 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas sp. DSM 6611 |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| Pisa1 forms a dimer. Each protomer consists of three domains: the N-terminal, catalytic alphabetabetaalpha sandwich domain, an alpha-helical dimerization domain and an alpha,beta-mixed C-terminal domain. Comparison with the structure of alkyl sulfatase SdsA1 | Pseudomonas sp. DSM 6611 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F250G | 190fold reduction in catalytic activity | Pseudomonas sp. DSM 6611 |
| H317A | 1300fold decrease in kcat, whereas Km is only slightly increased | Pseudomonas sp. DSM 6611 |
| S233F/F250G | three-fold to four-fold times lower kcat value and a 10fold higer Km value, variant displays activity towardstowards (S)-2-octyl sulfate | Pseudomonas sp. DSM 6611 |
| S233F/F250S | about 30% of wild-type activity | Pseudomonas sp. DSM 6611 |
| S233Y | 2.5fold reduction in catalytic activity | Pseudomonas sp. DSM 6611 |
| S233Y/F250G | three-fold to four-fold times lower kcat value | Pseudomonas sp. DSM 6611 |
| Y417D | inactive | Pseudomonas sp. DSM 6611 |
| Y417F | 114fold decrease in kcat value | Pseudomonas sp. DSM 6611 |
| Y417H | 238 fold decrease in kcat value | Pseudomonas sp. DSM 6611 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.099 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant S233Y, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 |  |
| 0.115 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant S233F/F250S, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 0.151 | - |
(2R)-octan-2-yl hydrogen sulfate | wild-type, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 0.24 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant H317A, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 1.5 | 2 | (2R)-octan-2-yl hydrogen sulfate | mutant S233Y/F250G, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 4.28 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant F250G, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 8.42 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant S233F/F250G, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | Pisa1 has a conserved binuclear zinc center | Pseudomonas sp. DSM 6611 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. DSM 6611 | F8KAY7 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2R)-octan-2-yl hydrogen sulfate + H2O | - |
Pseudomonas sp. DSM 6611 | (S)-2-octanol + sulfate | - |
? | |
| octyl sulfate + H2O | - |
Pseudomonas sp. DSM 6611 | 1-octanol + sulfate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pisa1 | - |
Pseudomonas sp. DSM 6611 |
| sec-alkylsulfatase | - |
Pseudomonas sp. DSM 6611 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant H317A, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 0.018 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant Y417H, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 0.02 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant F250G, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 0.038 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant Y417F, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 0.38 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant S233F/F250G, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 1.13 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant S233F/F250S, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 1.4 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant S233Y/F250G, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 1.73 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant S233Y, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 4.37 | - |
(2R)-octan-2-yl hydrogen sulfate | wild-type, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | Pisa1 shows a preference for secondary rather than primary alkyl sulfates, and enantioselectively hydrolyzes the (R)-enantiomer of rac-2-octyl sulfate, yielding (S)-2-octanol with inversion of absolute configuration as a result of C-O bond cleavage. An invariant histidine (His317) near the sulfate-binding site acts as general acid for crucial protonation of the sulfate leaving group | Pseudomonas sp. DSM 6611 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant F250G, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 0.014 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant H317A, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 0.046 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant S233F/F250G, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 0.919 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant S233Y/F250G, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 1.33 | - |
octyl sulfate | wild-type, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 9.85 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant S233F/F250S, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 17.5 | - |
(2R)-octan-2-yl hydrogen sulfate | mutant S233Y, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
| 28.9 | - |
(2R)-octan-2-yl hydrogen sulfate | wild-type, pH not specified in the publication, temperature not specified in the publication | Pseudomonas sp. DSM 6611 | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
