Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.6.19 extracted from

  • Shaw, D.J.; Dodgson, K.S.; White, G.F.
    Substrate specificity and other properties of the inducible S3 secondary alkylsulphohydrolase purified from the detergent-degrading bacterium Pseudomonas C12B (1980), Biochem. J., 187, 181-190.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.6.19

Inhibitors

Inhibitors Comment Organism Structure
decan-5-yl sulfate
-
 Pseudomonas sp.
K+
-
 Pseudomonas sp.
octan-5-yl sulfate
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 (5R)-nonan-5-yl sulfate pH 7.5, 30°C Pseudomonas sp.
15
-
 (4R)-heptan-4-yl sulfate pH 7.5, 30°C Pseudomonas sp.
40
-
 (3R)-pentan-3-yl sulfate pH 7.5, 30°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Pseudomonas sp.
46000
-
 polyacrylamide gel under non-denaturing conditions Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. C12B
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
124.3
-
 pH 7.5, 30°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R)-pentan-3-yl sulfate + H2O low activity Pseudomonas sp. pentan-3-ol + sulfate
-
 ?
(3R)-pentan-3-yl sulfate + H2O low activity Pseudomonas sp. C12B pentan-3-ol + sulfate
-
 ?
(4R)-heptan-4-yl sulfate + H2O the L-isomer is much more readily hydrolysed than the D-isomer. Enzymic hydrolysis of this group is accompanied by complete inversion of configuration at the asymmetric carbon atom Pseudomonas sp. (4S)-heptan-4-ol + sulfate
-
 ?
(4R)-heptan-4-yl sulfate + H2O the L-isomer is much more readily hydrolysed than the D-isomer. Enzymic hydrolysis of this group is accompanied by complete inversion of configuration at the asymmetric carbon atom Pseudomonas sp. C12B (4S)-heptan-4-ol + sulfate
-
 ?
(5R)-nonan-5-yl sulfate + H2O the L-isomer is much more readily hydrolysed than the D-isomer. Enzymic hydrolysis of this group is accompanied by complete inversion of configuration at the asymmetric carbon atom Pseudomonas sp. nonan-5-ol + sulfate
-
 ?
(5R)-nonan-5-yl sulfate + H2O the L-isomer is much more readily hydrolysed than the D-isomer. Enzymic hydrolysis of this group is accompanied by complete inversion of configuration at the asymmetric carbon atom Pseudomonas sp. C12B nonan-5-ol + sulfate
-
 ?

Synonyms

Synonyms Comment Organism
S3 secondary alkylsulphohydrolase
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pseudomonas sp.
8.2
-
 hydrolysis of heptan-4-yl sulfate and nonan-5-yl sulfate Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 9.2 5.0: about of 40% of maximal activity with nonan-5-yl sulfate, about 80% of maximal activity with heptan-4-yl sulfate. pH 9.2: about 55% of maximal activity with nonan-5-yl sulfate Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4
-
 decan-5-yl sulfate pH 7.5, 30°C Pseudomonas sp.
43
-
 octan-5-yl sulfate pH 7.5, 30°C Pseudomonas sp.