Literature summary for 3.1.6.1 extracted from

Zhu, Y.; Qiao, C.; Li, H.; Li, L.; Xiao, A.; Ni, H.; Jiang, Z.
Improvement thermostability of Pseudoalteromonas carrageenovora arylsulfatase by rational design (2018), Int. J. Biol. Macromol., 108, 953-959 .

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Protein Variants

Protein Variants	Comment	Organism
K253H/H260L	7.7fold increase in half-life compared to wild-type	Pseudoalteromonas carrageenovora
K253N	mutant shows enhanced thermal stability	Pseudoalteromonas carrageenovora
P314T	mutant shows enhanced thermal stability	Pseudoalteromonas carrageenovora

Organism

Organism	UniProt	Comment	Textmining
Pseudoalteromonas carrageenovora	P28607	-	-

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	-	30 min, 49% residual activtiy for mutant K253H, 33% for mutant P314T, 21% for wild-type, respectively. Half-life of wild-type 9.1 min, of mutant K253H/H260L 70.3 min	Pseudoalteromonas carrageenovora

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Release 2023.1 (January 2023)