Cloned (Comment) | Organism |
---|---|
expression in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C80A | about 20% of wild-type activity at neutral pH, about 5% of wild-type activity at pH optimum | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Homo sapiens | |
sulfate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.9 | - |
4-nitrocatechol sulfate | pH 4.6, 37°C | Homo sapiens | |
20.6 | - |
4-nitrocatechol sulfate | pH 4.6, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | protein carries mannose 6-phosphate, indicating lysosomal sorting | Homo sapiens | 5764 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
x * 68000, SDS-PAGE of recombinant glycoprotein, cellular form | Homo sapiens |
70000 | - |
x * 70000, SDS-PAGE of recombinant glycoprotein, secreted form | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6UWY0 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | when expressed in human cells, isoform ArsK is detected as a 68-kDa glycoprotein carrying at least four N-glycans of both the complex and high-mannose type. Protein carries mannose 6-phosphate, indicating lysosomal sorting | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | mRNA expression is found in all tissues tested | Homo sapiens | - |
pancreas | high level of expression | Homo sapiens | - |
placenta | high level of expression | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrocatechol sulfate + H2O | - |
Homo sapiens | 4-nitrocatechol + sulfate | - |
? | |
4-nitrophenyl sulfate + H2O | - |
Homo sapiens | 4-nitrophenol + sulfate | - |
? | |
additional information | activity is dependent on residue cysteine80, which undergoes conversion to formylglycine. No substrate: 4-methylumbelliferyl sulfate | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68000, SDS-PAGE of recombinant glycoprotein, cellular form | Homo sapiens |
? | x * 70000, SDS-PAGE of recombinant glycoprotein, secreted form | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ARSK | - |
Homo sapiens |
arylsulfatase K | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.6 | - |
- |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
2.4 | - |
pH 4.6, 37°C | Homo sapiens | phosphate | |
2.9 | - |
pH 4.6, 37°C | Homo sapiens | sulfate |