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Literature summary for 3.1.6.1 extracted from

  • Frese, M.A.; Schulz, S.; Dierks, T.
    Arylsulfatase G, a novel lysosomal sulfatase (2008), J. Biol. Chem., 283, 11388-11395.
    View publication on PubMed
Search for more literature for 3.1.6.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HT-1080 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information ASG is not inhibited by warfarin even at concentrations close to the solubility limit Homo sapiens
phosphate acts as a strong, competitive ASG inhibitor, inhibition of ASG by phosphate is much stronger than by sulfate Homo sapiens
sulfate
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens ASG does not undergo lysosomal processing ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein ASG is a glycoprotein that binds specifically to mannose 6-phosphate receptors, the activity of arylsulfatase G depends on the Cys84 residue that is predicted to be posttranslationally converted to the critical active site Calpha-formylglycine Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and HisTrap column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
kidney highest expression Homo sapiens
-
liver highest expression Homo sapiens
-
pancreas highest expression Homo sapiens
-
placenta
-
 Homo sapiens
-
skeletal muscle
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
4°C, pH 6.0, half-life of more than 100 days Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl sulfate + H2O rather poor substrate Homo sapiens 4-methylumbelliferol + sulfate
-
 ?
4-nitrocatechol sulfate + H2O best substrate Homo sapiens 4-nitrocatechol + sulfate
-
 ?
4-nitrophenyl sulfate + H2O
-
 Homo sapiens 4-nitrophenol + sulfate
-
 ?
additional information ASG does not undergo lysosomal processing Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 63000, active enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
arylsulfatase G
-
 Homo sapiens
ASG
-
 Homo sapiens
KIAA1001
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50 ASG cleaves 4-nitrocatechol sulfate most efficiently at 45-50°C Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 ASG activity is retained even at relatively high temperatures above 55°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
 for the reaction with 4-nitrocatechol sulfate Homo sapiens
5.6
-
 for the reaction with 4-methylumbelliferyl sulfate Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.001
-
 at 10 mM 4-nitrocatechol sulfate Homo sapiens sulfate
0.05
-
 at 10 mM 4-nitrocatechol sulfate Homo sapiens phosphate