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Literature summary for 3.1.6.1 extracted from

  • Olguin, L.F.; Askew, S.E.; ODonoghue, A.C.; Hollfelder, F.
    Efficient catalytic promiscuity in an enzyme superfamily: An arylsulfatase shows a rate acceleration of 10(13) for phosphate monoester hydrolysis (2008), J. Am. Chem. Soc., 130, 16547-16555.
    View publication on PubMed
Search for more literature for 3.1.6.1

Protein Variants

Protein Variants Comment Organism
C51A mutant with strongly reduced activity Pseudomonas aeruginosa
C51S mutant with strongly reduced activity Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
4-nitrophenyl phosphate
-
 Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00025
-
 4-nitrophenyl sulfate mutant enzyme C51S, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa
0.00029
-
 4-nitrophenyl sulfate wild type enzyme, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa
0.0004
-
 4-nitrophenyl sulfate mutant enzyme C51A, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa
0.0291
-
 4-nitrophenyl phosphate wild type enzyme, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa
0.0752
-
 4-nitrophenyl phosphate mutant enzyme C51S, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 SDS-PAGE Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl sulfate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + sulfate
-
 ?

Synonyms

Synonyms Comment Organism
PAS
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000095
-
 4-nitrophenyl phosphate mutant enzyme C51S, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa
0.000061
-
 4-nitrophenyl sulfate mutant enzyme C51A, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa
0.0054
-
 4-nitrophenyl sulfate mutant enzyme C51S, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa
0.023
-
 4-nitrophenyl phosphate wild type enzyme, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa
14.2
-
 4-nitrophenyl sulfate wild type enzyme, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0292
-
 4-nitrophenyl phosphate wild type enzyme, in 100 mM Tris, pH 8.0, 25°C Pseudomonas aeruginosa