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Literature summary for 3.1.6.1 extracted from

  • Clark, A.G.; Jowett, D.A.
    Purification and properties of an arylsulphatase from the digestive gland of Haliotis iris (1988), Comp. Biochem. Physiol. B, 89, 409-417.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2-hydroxy-4-methyl-5-nitrophenyl sulfate substrate inhibition Haliotis iris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
2-hydroxy-5-nitrophenyl sulfate in presence of 400 mM acetate Haliotis iris
0.0486
-
2-hydroxy-5-nitrophenyl sulfate in presence of 25-400 mM acetate Haliotis iris
0.085
-
2-hydroxy-5-nitrophenyl sulfate in presence of 100 mM acetate and 100 mM sulfate Haliotis iris
0.123
-
4-hydroxy-2-nitrophenyl sulfate in presence of 100 mM acetate Haliotis iris
0.181
-
2-hydroxy-4-nitrophenyl sulfate in presence of 100 mM acetate Haliotis iris
0.193
-
4-hydroxy-3-nitrophenyl sulfate in presence of 100 mM acetate Haliotis iris

Organism

Organism UniProt Comment Textmining
Haliotis iris
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Haliotis iris

Purification (Commentary)

Purification (Comment) Organism
-
Haliotis iris

Source Tissue

Source Tissue Comment Organism Textmining
digestive gland
-
Haliotis iris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Haliotis iris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-4-nitrophenyl sulfate + H2O
-
Haliotis iris 2-hydroxy-4-nitrophenol + sulfate
-
?
2-hydroxy-5-nitrophenyl sulfate + H2O
-
Haliotis iris 2-hydroxy-5-nitrophenol + sulfate
-
?
4-hydroxy-2-nitrophenyl sulfate + H2O
-
Haliotis iris 4-hydroxy-2-nitrophenol + sulfate
-
?
4-hydroxy-3-nitrophenyl sulfate + H2O
-
Haliotis iris 4-hydroxy-3-nitrophenol + sulfate
-
?
nitrocatechol sulfate + H2O
-
Haliotis iris nitrocatechol + sulfate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 hydrolysis of 2-hydroxy-5-nitrophenyl sulfate and 2-hydroxy-4-nitrophenyl sulfate Haliotis iris