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Literature summary for 3.1.6.1 extracted from

  • Okamura, H.; Yamada, T.; Murooka, Y.; Harada, T.
    Purification and properties of arylsulfatase of Klebsiella aerogenes. Identity of the enzymes formed by non-repressed and de-repressed synthesis (1976), Agric. Biol. Chem., 40, 2071-2076.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
CN- KCN Klebsiella aerogenes
dopamine
-
Klebsiella aerogenes
hydroxylamine
-
Klebsiella aerogenes
Na2SO3
-
Klebsiella aerogenes
octopamine
-
Klebsiella aerogenes
PO43-
-
Klebsiella aerogenes
tyramine
-
Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
NH4+ 25 mM, 60% activation Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
gel filtration Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-
Klebsiella aerogenes W70
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
56
-
-
Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl sulfate + H2O
-
Klebsiella aerogenes 2-nitrophenol + sulfate
-
?
2-nitrophenyl sulfate + H2O
-
Klebsiella aerogenes W70 2-nitrophenol + sulfate
-
?
3-carboxyphenyl sulfate + H2O weak activity Klebsiella aerogenes 3-carboxyphenol + sulfate
-
?
3-carboxyphenyl sulfate + H2O weak activity Klebsiella aerogenes W70 3-carboxyphenol + sulfate
-
?
3-nitrophenyl sulfate + H2O
-
Klebsiella aerogenes 3-nitrophenol + sulfate
-
?
3-nitrophenyl sulfate + H2O
-
Klebsiella aerogenes W70 3-nitrophenol + sulfate
-
?
4-nitrocatechol sulfate + H2O
-
Klebsiella aerogenes 4-nitrocatechol + sulfate
-
?
4-nitrocatechol sulfate + H2O
-
Klebsiella aerogenes W70 4-nitrocatechol + sulfate
-
?
4-nitrophenyl sulfate + H2O
-
Klebsiella aerogenes 4-nitrophenol + sulfate
-
?
4-nitrophenyl sulfate + H2O
-
Klebsiella aerogenes W70 4-nitrophenol + sulfate
-
?
indoxyl sulfate + H2O weak activity Klebsiella aerogenes indoxol + sulfate
-
?
phenyl sulfate + H2O weak activity Klebsiella aerogenes phenol + sulfate
-
?
Tyr-O-sulfate + H2O weak activity Klebsiella aerogenes Tyr + sulfate
-
?
tyramine-O-sulfate + H2O weak activity Klebsiella aerogenes tyramine + sulfate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Klebsiella aerogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
5 min, about 50% loss of activity Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Klebsiella aerogenes