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Literature summary for 3.1.5.1 extracted from

  • Quirk, S.; Do, B.T.
    Cloning, purification, and characterization of the Shigella boydii dGTP triphosphohydrolase (1997), J. Biol. Chem., 272, 332-336.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Shigella boydii

Crystallization (Commentary)

Crystallization (Comment) Organism
initial conditions, vapor diffusion method Shigella boydii

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol prior incubation, loss of thermostability Shigella boydii
dithiothreitol prior incubation, 12% of activity remains, loss of thermostability Shigella boydii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59400
-
 homotetramer, 4 * 59400 Shigella boydii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dGTP + H2O Shigella boydii
-
 deoxyguanosine + triphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Shigella boydii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein to homogeneity Shigella boydii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dGTP + H2O
-
 Shigella boydii deoxyguanosine + triphosphate
-
 ?
dGTP + H2O highly specific for Shigella boydii deoxyguanosine + triphosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer homotetramer, 4 * 59400 Shigella boydii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 loss of thermostability in presence of reducing agents, 5% of activity remains Shigella boydii