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Literature summary for 3.1.5.1 extracted from

  • Beauchamp, B.B.; Richardson, Ch.C.
    A unique deoxyguanosine triphosphatase is responsible for the optA1 phenotype of Escherichia coli (1988), Proc. Natl. Acad. Sci. USA, 85, 2563-2567.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.5.1

General Stability

General Stability Organism
more stable at higher protein concentrations Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002 0.005 dGTP
-
 Escherichia coli
0.15
-
 GTP
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dGTP + H2O Escherichia coli
-
 deoxyguanosine + triphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 mutant optA1
-

Purification (Commentary)

Purification (Comment) Organism
16000 fold to homogeneity Escherichia coli
high affinity for DNA Escherichia coli

Storage Stability

Storage Stability Organism
0 to -20°C, 0.1 mg/ml protein concentration, 30 days, 20% and 60% loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dGTP + H2O
-
 Escherichia coli deoxyguanosine + triphosphate
-
 ?
dGTP + H2O highly specific for Escherichia coli deoxyguanosine + triphosphate
-
 ?
dGTP + H2O no activity on dGDP Escherichia coli deoxyguanosine + triphosphate
-
 ?
GTP + H2O
-
 Escherichia coli guanosine + triphosphate
-
 ?

Synonyms

Synonyms Comment Organism
More 50 fold increased level Escherichia coli
More C-T transition 74 bases upstream Escherichia coli
More OPTA1 mutants Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 reversible loss of activity at low protein concentrations Escherichia coli
55 60 full activity after 1 h Escherichia coli
65 70 90% denatured after 1 h Escherichia coli