KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
dGTP | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activation 17% the rate of Mg2+ | Escherichia coli | |
Mg2+ | no activity in absence of | Escherichia coli | |
Mn2+ | activation 30% the rate of Mg2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58900 | - |
homotetramer, 4 * 58900 | Escherichia coli |
230000 | - |
gel filtration | Escherichia coli |
230000 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dGTP + H2O | Escherichia coli | - |
deoxyguanosine + triphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
16000 fold to homogeneity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dGTP + H2O | - |
Escherichia coli | deoxyguanosine + triphosphate | - |
? | |
dGTP + H2O | highly specific for | Escherichia coli | deoxyguanosine + triphosphate | - |
? | |
dGTP + H2O | triphosphate group required | Escherichia coli | deoxyguanosine + triphosphate | - |
? | |
dGTP + H2O | no activity on dGDP | Escherichia coli | deoxyguanosine + triphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | SDS-PAGE | Escherichia coli |
tetramer | homotetramer, 4 * 58900 | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | full activity after 1 h | Escherichia coli |
65 | 70 | 90% denatured after 1 h | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4000 | - |
dGTP | - |
Escherichia coli |