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Literature summary for 3.1.4.54 extracted from
- Molecular characterization of a phospholipase D generating anandamide and its congeners (2003), J. Biol. Chem., 279, 5298-5305.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in COS-7 cells | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0028 | - |
N-arachidonoyl-1,2-dioleoylphosphatidylethanolamine | pH 7.5, 37°C | Rattus norvegicus |  |
| 0.0029 | - |
N-oleoyl-1,2-dioleoylphosphatidylethanolamine | pH 7.5, 37°C | Rattus norvegicus | |
| 0.0033 | - |
N-palmitoyl-1-palmitoyl-2-linoleoylphosphatidylethanolamine | pH 7.5, 37°C | Rattus norvegicus | |
| 0.0034 | - |
N-stearoyl-1,2-dioleoylphosphatidylethanolamine | pH 7.5, 37°C | Rattus norvegicus | |
| 0.004 | - |
N-palmitoyl-1-palmitoyl-2-lysophosphatidylethanolamine | pH 7.5, 37°C | Rattus norvegicus | |
| 0.004 | - |
N-arachidonoyl-1-oleoyl-2-lysophosphatidylethanolamine | pH 7.5, 37°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rattus norvegicus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | member of the zinc metallohydrolase family of the beta-lactamase fold | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | Q769K2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| testis | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0979 | - |
substrate: N-palmitoyl-1-palmitoyl-2-linoleoyl-phosphatidylethanolamine, pH 7.5, 37°C | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is inactive with phosphatidylcholine and phosphatidylethanolamine. No transphosphatidation | Rattus norvegicus | ? | - |
? | |
| N-arachidonoyl-1,2-dioleoylphosphatidylethanolamine + H2O | - |
Rattus norvegicus | N-arachidonoylethanolamine + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
| N-arachidonoyl-1-oleoyl-2-lysophosphatidylethanolamine + H2O | - |
Rattus norvegicus | N-arachidonoylethanolamine + 1-oleoyl-2-lyso-sn-glycerol 3-phosphate | - |
? | |
| N-oleoyl-1,2-dioleoylphosphatidylethanolamine + H2O | - |
Rattus norvegicus | N-oleoylethanolamine + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
| N-palmitoyl-1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O | - |
Rattus norvegicus | N-palmitoylethanolamine + 1-palmitoyl-2-linoleoyl-sn-glycerol 3-phosphate | - |
? | |
| N-palmitoyl-1-palmitoyl-2-lysophosphatidylethanolamine + H2O | - |
Rattus norvegicus | N-palmitoylethanolamine + 1-palmitoyl-2-lyso-sn-glycerol 3-phosphate | - |
? | |
| N-stearoyl-1,2-dioleoylphosphatidylethanolamine + H2O | - |
Rattus norvegicus | N-stearoylethanolamine + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAPE-PLD | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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