Cloned (Comment) | Organism |
---|---|
- |
Mus musculus |
- |
Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C224S | considerable reduction in activity | Rattus norvegicus |
H380R | considerable reduction in activity | Rattus norvegicus |
L207F | considerable reduction in activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | presence of of catalytically crucial Zn2+, NAPE-PLD is a member of the metallo-beta-lactamase family | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-arachidonoyl-phosphatidylethanolamine + H2O | Mus musculus | the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines. Analysis of NAPE-PLD-deficient mice reveals the presence of NAPE-PLD independent pathways for the anandamide formation | N-arachidonoylethanolamine + phosphatidic acid | N-arachidonoylethanolamine i.e. anandamide | ? | |
N-arachidonoylphosphatidylethanolamine + H2O | Rattus norvegicus | the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines | N-arachidonoylethanolamine + phosphatidic acid | N-arachidonoylethanolamine i.e. anandamide | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6IQ20 | - |
- |
Mus musculus | Q8BH82 | - |
- |
Rattus norvegicus | Q769K2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | activity detected in almost all of the mouse organs, with higher specific activities in brain, kidney, and testis | Mus musculus | - |
brain | widely distributed in various brain regions of rat, with the highest activity in the brain stem. The enzyme activity in rat brain changes during development, and infant rats show a several-fold higher activity than adult rats | Rattus norvegicus | - |
kidney | activity detected in almost all of the mouse organs, with higher specific activities in brain, kidney, and testis | Mus musculus | - |
oviduct | NAPE-PLD is expressed in mouse oviduct on days one to four of pregnancy | Mus musculus | - |
testis | - |
Rattus norvegicus | - |
testis | activity detected in almost all of the mouse organs, with higher specific activities in brain, kidney, and testis | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-arachidonoyl-phosphatidylethanolamine + H2O | the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines. Analysis of NAPE-PLD-deficient mice reveals the presence of NAPE-PLD independent pathways for the anandamide formation | Mus musculus | N-arachidonoylethanolamine + phosphatidic acid | N-arachidonoylethanolamine i.e. anandamide | ? | |
N-arachidonoylphosphatidylethanolamine + H2O | the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines | Rattus norvegicus | N-arachidonoylethanolamine + phosphatidic acid | N-arachidonoylethanolamine i.e. anandamide | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
NAPE-PLD | - |
Mus musculus |
NAPE-PLD | - |
Homo sapiens |
NAPE-PLD | - |
Rattus norvegicus |
Organism | Comment | Expression |
---|---|---|
Rattus norvegicus | the enzyme appears to be constitutively active | additional information |
General Information | Comment | Organism |
---|---|---|
malfunction | the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines. Analysis of NAPE-PLD-deficient mice reveals the presence of NAPE-PLD independent pathways for the anandamide formation | Mus musculus |
physiological function | the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines. Analysis of NAPE-PLD-deficient mice reveals the presence of NAPE-PLD independent pathways for the anandamide formation | Mus musculus |