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Literature summary for 3.1.4.54 extracted from

  • Okamoto, Y.; Wang, J.; Morishita, J.; Ueda, N.
    Biosynthetic pathways of the endocannabinoid anandamide (2007), Chem. Biodivers., 4, 1842-1857.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Mus musculus
-
Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
C224S considerable reduction in activity Rattus norvegicus
H380R considerable reduction in activity Rattus norvegicus
L207F considerable reduction in activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate
-
Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ presence of of catalytically crucial Zn2+, NAPE-PLD is a member of the metallo-beta-lactamase family Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
x * 46000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-arachidonoyl-phosphatidylethanolamine + H2O Mus musculus the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines. Analysis of NAPE-PLD-deficient mice reveals the presence of NAPE-PLD independent pathways for the anandamide formation N-arachidonoylethanolamine + phosphatidic acid N-arachidonoylethanolamine i.e. anandamide ?
N-arachidonoylphosphatidylethanolamine + H2O Rattus norvegicus the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines N-arachidonoylethanolamine + phosphatidic acid N-arachidonoylethanolamine i.e. anandamide ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6IQ20
-
-
Mus musculus Q8BH82
-
-
Rattus norvegicus Q769K2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain activity detected in almost all of the mouse organs, with higher specific activities in brain, kidney, and testis Mus musculus
-
brain widely distributed in various brain regions of rat, with the highest activity in the brain stem. The enzyme activity in rat brain changes during development, and infant rats show a several-fold higher activity than adult rats Rattus norvegicus
-
kidney activity detected in almost all of the mouse organs, with higher specific activities in brain, kidney, and testis Mus musculus
-
oviduct NAPE-PLD is expressed in mouse oviduct on days one to four of pregnancy Mus musculus
-
testis
-
Rattus norvegicus
-
testis activity detected in almost all of the mouse organs, with higher specific activities in brain, kidney, and testis Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-arachidonoyl-phosphatidylethanolamine + H2O the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines. Analysis of NAPE-PLD-deficient mice reveals the presence of NAPE-PLD independent pathways for the anandamide formation Mus musculus N-arachidonoylethanolamine + phosphatidic acid N-arachidonoylethanolamine i.e. anandamide ?
N-arachidonoylphosphatidylethanolamine + H2O the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines Rattus norvegicus N-arachidonoylethanolamine + phosphatidic acid N-arachidonoylethanolamine i.e. anandamide ?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
NAPE-PLD
-
Mus musculus
NAPE-PLD
-
Homo sapiens
NAPE-PLD
-
Rattus norvegicus

Expression

Organism Comment Expression
Rattus norvegicus the enzyme appears to be constitutively active additional information

General Information

General Information Comment Organism
malfunction the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines. Analysis of NAPE-PLD-deficient mice reveals the presence of NAPE-PLD independent pathways for the anandamide formation Mus musculus
physiological function the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines. Analysis of NAPE-PLD-deficient mice reveals the presence of NAPE-PLD independent pathways for the anandamide formation Mus musculus