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Literature summary for 3.1.4.53 extracted from

  • Zhu, S.; Gan, Z.; Li, Z.; Liu, Y.; Yang, X.; Deng, P.; Xie, Y.; Yu, M.; Liao, H.; Zhao, Y.; Zhao, L.; Liao, F.
    The measurement of cyclic nucleotide phosphodiesterase 4 activities via the quantification of inorganic phosphate with malachite green (2009), Anal. Chim. Acta, 636, 105-110.
    View publication on PubMed
Search for more literature for 3.1.4.53

Activating Compound

Activating Compound Comment Organism Structure
EDTA EDTA at 0.10 mM slightly activates PDE4 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information not affected by Ca2+, Fe2+, Mn2+, HSCH2CH2OH, methanol, and dimethylsulfoxide Homo sapiens
rolipram
-
 Homo sapiens
Zn2+ more than 90% inhibition at 0.05 mM Zn2+ in the presence of 0.1 mM EDTA, inhibition can be greatly relieved with EDTA at 0.30 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0088
-
 3',5'-cAMP
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation and amylose-resin column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3',5'-cAMP + H2O
-
 Homo sapiens 5'-AMP
-
 ?

Synonyms

Synonyms Comment Organism
cyclic nucleotide phosphodiesterase 4
-
 Homo sapiens
PDE4
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00001
-
 in 20 mM Tris-HCl at pH 7.4, 10 mM MgCl2, and 0.1 mM EDTA, at 30°C Homo sapiens rolipram