Literature summary for 3.1.4.53 extracted from

Fisher, D.A.; Smith, J.F.; Pillar, J.S.; Denis, S.H.St.; Cheng, J.B.
Isolation and characterization of PDE8A, a novel human cAMP-specific phosphodiesterase (1998), Biochem. Biophys. Res. Commun., 246, 570-577.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in baculovirus transfected Sf9 cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
dipyridimole	-	Homo sapiens
additional information	the enzyme is insensitive (up to 100 mM) to a variety of PDE inhibitors including rolipram, zaprinast, vinpocetine, SKF-94120, and IBMX	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000055	-	adenosine 3',5'-cyclic phosphate	-	Homo sapiens
0.124	-	guanosine 3',5'-cyclic phosphate	-	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	the enzyme requires the presence of at least 1 mM Mn2+ or Mg2+ for maximal activity in vitro	Homo sapiens
Mn2+	the enzyme requires the presence of at least 1 mM Mn2+ or Mg2+ for maximal activity in vitro	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O60658	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
colon	-	Homo sapiens	-
ovary	-	Homo sapiens	-
small intestine	-	Homo sapiens	-
testis	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
adenosine 3',5'-cyclic phosphate + H2O	-	Homo sapiens	adenosine 5'-phosphate	-	?
guanosine 3',5'-cyclic phosphate + H2O	-	Homo sapiens	guanosine 5'-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
cAMP-specific phosphodiesterase	-	Homo sapiens
high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A	-	Homo sapiens
PDE8A	-	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.009	-	-	Homo sapiens	dipyridimole

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Release 2023.1 (January 2023)