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Literature summary for 3.1.4.53 extracted from

  • Mackenzie, K.F.; Topping, E.C.; Bugaj-Gaweda, B.; Deng, C.; Cheung, Y.F.; Olsen, A.E.; Stockard, C.R.; Mitchell, L.H.; Baillie, G.S.; Grizzle, W.E.; Devivo, M.; Houslay, M.D.; Wang, D.; Bolger, G.B.
    Human PDE4A8, a novel brain-expressed PDE4 cAMP-specific phosphodiesterase that has undergone rapid evolutionary change (2007), Biochem. J., 411, 361-369.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS-7 cell Homo sapiens
When expressed in COS-7 cells, PDE4A8 localizes predominantly in the cytosol, but 20% of the enzyme was associated with membrane fractions Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
cilomilast
-
Homo sapiens
rolipram
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol predominant localization, recombinant protein expressed in COS-7 cell Homo sapiens 5829
-
cytosol when expressed in COS-7 cells, PDE4A8 localizes predominantly in the cytosol, but 20% of the enzyme is associated with membrane fractions Homo sapiens 5829
-
membrane about 20% of protein is associated with membrane, recombinant protein expressed in COS-7 cell Homo sapiens 16020
-
membrane when expressed in COS-7 cells, PDE4A8 localizes predominantly in the cytosol, but 20% of the enzyme is associated with membrane fractions Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
125000
-
x * 125000, SDS-PAGE, recombinant protein Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P27815
-
-
Homo sapiens P27815 isoform PDE4A8
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation of enzyme by cAMP-dependent protein kinase at S123 Homo sapiens
phosphoprotein phosphorylation by cAMP-dependent protein kinase Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-
brain and skeletal muscle, predominant expression Homo sapiens
-
cerebellum
-
Homo sapiens
-
cerebral cortex
-
Homo sapiens
-
skeletal muscle
-
Homo sapiens
-
skeletal muscle and brain, predominant expression Homo sapiens
-
spinal cord
-
Homo sapiens
-
testis
-
Homo sapiens
-

Subunits

Subunits Comment Organism
? x * 125000, SDS-PAGE, recombinant protein Homo sapiens

Synonyms

Synonyms Comment Organism
PDE4A8
-
Homo sapiens
phosphodiesterase 4 isoform A8
-
Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000011
-
-
Homo sapiens rolipram
0.000101
-
-
Homo sapiens cilomilast