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Literature summary for 3.1.4.49 extracted from

  • Tomita, Y.; Motokawa, Y.
    Characterization and partial purification of a novel mannosylphosphodolichol phosphodiesterase from chicken liver microsomes (1987), Eur. J. Biochem., 170, 363-368.
    View publication on PubMed
Search for more literature for 3.1.4.49

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol requirement, or other SH-reducing agents Gallus gallus
Emulgen 909 requirement, optimum concentration: 0.4% v/v Gallus gallus
additional information no requirement for any phospholipid Gallus gallus
SH-reducing agents e.g. 2-mercaptoethanol, absolute requirement Gallus gallus

General Stability

General Stability Organism
a heat-stable factor, precipitable by trichloroacetic acid, insoluble in lipid solvents, stabilizes, separation from this factor by ion-exchange chromatography inactivates irreversibly Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
bis(4-nitrophenyl)phosphate 1 mM, competitive inhibition Gallus gallus
dolichol 0.1 mM, strong, competitive inhibition Gallus gallus
dolichyl phosphate 0.1 mM, strong, competitive inhibition Gallus gallus
additional information not inhibited by D-mannose, mannose 1-phosphate, GDP, GMP, ADP, AMP, phosphate, diphosphate GDPmannose, UDP-N-acetylglucosamine, UDPglucose, UDPglucuronic acid, 4-nitrophenyl phosphate, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside or 4-nitrophenyl-beta-D-glucopyranoside Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00043
-
 Mannosylphosphodolichol pH 5.3, 37°C Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-
microsome predominantly Gallus gallus
-
-
mitochondrion
-
 Gallus gallus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 greatly stimulates, optimum concentration: 2 mM Gallus gallus
additional information not activated by other divalent cations than Ca2+ Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
mannosylphosphodolichol + H2O Gallus gallus enzyme may be capable of degrading Dol-P-Man in vivo D-mannose + dolichyl phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, 57.9fold, solubilized with 1% v/v Emulgen 909 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Gallus gallus
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00000011
-
 pH 6.3 Rattus norvegicus
0.000631
-
 pH 5.3, 37°C Gallus gallus

Storage Stability

Storage Stability Organism
0°C, in the presence of 2-mercaptoethanol and stabilizing factor, 1 month, stable Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mannosylphosphodolichol + H2O
-
 Rattus norvegicus D-mannose + dolichyl phosphate
-
 ?
mannosylphosphodolichol + H2O highly specific for Dol-P-Man Gallus gallus D-mannose + dolichyl phosphate
-
 ?
mannosylphosphodolichol + H2O enzyme may be capable of degrading Dol-P-Man in vivo Gallus gallus D-mannose + dolichyl phosphate
-
 ?
additional information not: N-acetylglucosaminyldiphosphodolichol, glucosylphosphodolichol or mannose 1-phosphate, artificial substrates for acid phosphatase, acid phosphodiesterase, mannosidase or glucosidase Gallus gallus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3
-
 acetate buffer Gallus gallus
6.3
-
 assay at Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0125
-
 dolichyl phosphate pH 5.3, 37°C Gallus gallus