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Literature summary for 3.1.4.46 extracted from

  • Wang, F.; Lai, L.; Liu, Y.; Yang, B.; Wang, Y.
    Expression and characterization of a novel glycerophosphodiester phosphodiesterase from Pyrococcus furiosus DSM 3638 that possesses lysophospholipase D activity (2016), Int. J. Mol. Sci., 17, 831 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.46

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of structure and docking of substrates. Residues Glu44, Asp46, and Glu109 constitute a metal-binding cleft, with a Mg2+ ion bound Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ optimum concentration 5 mM Pyrococcus furiosus
Mn2+ optimum concentration 5 mM Pyrococcus furiosus
additional information divalent cation required, best activators are Co2+ and Mn2+. Poor or no activation by Cu2+, Mg2+, Ca2+, Li+, Fe3+, Zn2+ Pyrococcus furiosus
Ni2+ about 40% of the activity with Co2+ or Mn2+ Pyrococcus furiosus

Organic Solvent Stability

Organic Solvent Comment Organism
chloroform 50% v/v, 75% residual activity Pyrococcus furiosus
dichloromethane 50% v/v, 95% residual activity Pyrococcus furiosus
diethyl ether 50% v/v, 59% residual activity Pyrococcus furiosus
Ethyl acetate 50% v/v, 90% residual activity Pyrococcus furiosus
hexane 50% v/v, 85% residual activity Pyrococcus furiosus
toluene 50% v/v, 85% residual activity Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8TZI9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-lysophosphatidylcholine + H2O poor substrate in comparison to glycerophosphocholine Pyrococcus furiosus choline + 1-lysophosphatidate
-
 ?
2-lysophosphatidylcholine + H2O very poor substrate in comparison to glycerophosphocholine Pyrococcus furiosus choline + 2-lysophosphatidate
-
 ?
bis(p-nitrophenyl) phosphate + H2O
-
 Pyrococcus furiosus p-nitrophenyl phosphate + p-nitrophenol
-
 ?
glycerophosphocholine + H2O
-
 Pyrococcus furiosus choline + glycerol-3-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 34059, calculated from sequence Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
Pf2003
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80
-
 almost complete loss of activity Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life 17.6 h Pyrococcus furiosus
59.6
-
 melting temperature Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
7 10 no activity below pH 7.0 Pyrococcus furiosus