Cloned (Comment) | Organism |
---|---|
generation of an 86-028NP-derived NTHI strain that expresses an hpd/glpQ promoter-driven luciferase reporter from the vector pKMLN-01, hpd/glpQ is expressed in an operon with glpT | Haemophilus influenzae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a nonpolar hpd/glpQ mutant strain, that retains detectable mRNA expression of the upstream gene glpT, which is cotranscribed with hpd/glpQ | Haemophilus influenzae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
outer membrane | - |
Haemophilus influenzae | 19867 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alpha-glycerophosphocholine + H2O | Haemophilus influenzae | - |
choline + sn-glycerol-3-phosphate | - |
? | |
L-alpha-glycerophosphocholine + H2O | Haemophilus influenzae 86-028NP | - |
choline + sn-glycerol-3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
nontypeable Haemophilus influenzae | - |
Haemophilus influenzae 86-028NP | - |
nontypeable Haemophilus influenzae | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
lipoprotein | - |
Haemophilus influenzae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epithelial cell | - |
Haemophilus influenzae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alpha-glycerophosphocholine + H2O | - |
Haemophilus influenzae | choline + sn-glycerol-3-phosphate | - |
? | |
L-alpha-glycerophosphocholine + H2O | - |
Haemophilus influenzae 86-028NP | choline + sn-glycerol-3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PD/GlpQ | - |
Haemophilus influenzae |
Protein D | - |
Haemophilus influenzae |
protein D/glycerophosphodiester phosphodiesterase | - |
Haemophilus influenzae |
General Information | Comment | Organism |
---|---|---|
malfunction | abrogation of nontypeable Haemophilus influenzae protein D function reduces phosphorylcholine decoration, adherence to airway epithelial A549 cells, and fitness in a chinchilla, Chinchilla lanigera, model of otitis media. Exposure of NTHI to antibodies directed against the vaccine formulation recapitulates the phosphorylcholine decoration and NTHI adherence phenotypes exhibite by PD/GlpQ-deficient NTHI, mechanism(s) of anti-PD/GlpQ antibody-mediated protection from NTHI-induced otitis media, overview | Haemophilus influenzae |
physiological function | PD/GlpQ enzymatic activity is predicted to facilitate choline and sn-glycerol-3-phosphate acquisition via the cleavage of L-alpha-glycerophosphocholine, an abundant catabolite of host phospholipids | Haemophilus influenzae |