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Literature summary for 3.1.4.46 extracted from

  • Jackson, C.J.; Hadler, K.S.; Carr, P.D.; Oakley, A.J.; Yip, S.; Schenk, G.; Ollis, D.L.
    Malonate-bound structure of the glycerophosphodiesterase from Enterobacter aerogenes (GpdQ) and characterization of the native Fe2+ metal-ion preference (2008), Acta Crystallogr. Sect. F, 64, 681-685.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Klebsiella aerogenes

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5
-
 bis(p-nitrophenyl phosphate) in the presence of 0.1 mM Zn2+ Klebsiella aerogenes
7
-
 bis(p-nitrophenyl phosphate) in the presence of 0.1 mM Fe2+ Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes Q6XBH1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bis(p-nitrophenyl) phosphate + H2O
-
 Klebsiella aerogenes p-nitrophenyl phosphate + p-nitrophenol
-
 ?

Synonyms

Synonyms Comment Organism
glycerophosphodiesterase
-
 Klebsiella aerogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.176
-
 bis(p-nitrophenyl phosphate) in the presence of 0.1 mM Zn2+ Klebsiella aerogenes
2.78
-
 bis(p-nitrophenyl phosphate) in the presence of 0.1 mM Fe2+ Klebsiella aerogenes

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0498
-
 bis(p-nitrophenyl phosphate) in the presence of 0.1 mM Zn2+ Klebsiella aerogenes
0.4
-
 bis(p-nitrophenyl phosphate) in the presence of 0.1 mM Fe2+ Klebsiella aerogenes