Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Caldanaerobacter subterraneus subsp. tengcongensis |
Crystallization (Comment) | Organism |
---|---|
crystal structure of the Thermoanaerobacter tengcongensis (ttGDPD) at 1.91 A resolution is reported, with a calcium ion and glycerol as a substrate mimic coordinated at this calcium ion (PDB entry 2pz0). The ttGDPD dimer with an intermolecular disulfide bridge and two hydrogen bonds is considered as the potential functional unit | Caldanaerobacter subterraneus subsp. tengcongensis |
Protein Variants | Comment | Organism |
---|---|---|
D46A | mutant shows no activity | Caldanaerobacter subterraneus subsp. tengcongensis |
E119A | mutant shows no activity | Caldanaerobacter subterraneus subsp. tengcongensis |
E44A | mutant shows no activity | Caldanaerobacter subterraneus subsp. tengcongensis |
H59A | relative activity: 1.39% | Caldanaerobacter subterraneus subsp. tengcongensis |
K121A | relative activity: 4.7% | Caldanaerobacter subterraneus subsp. tengcongensis |
R18A | relative activity: 10.7% | Caldanaerobacter subterraneus subsp. tengcongensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM CaCl2, relative activity: 28.5% | Caldanaerobacter subterraneus subsp. tengcongensis | |
Mg2+ | 10 mM MgCl2, relative activity: 100% | Caldanaerobacter subterraneus subsp. tengcongensis | |
additional information | enzyme requires a divalent metal cation | Caldanaerobacter subterraneus subsp. tengcongensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldanaerobacter subterraneus subsp. tengcongensis | Q8RB32 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography and gel filtration | Caldanaerobacter subterraneus subsp. tengcongensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerophosphorylcholine + H2O | - |
Caldanaerobacter subterraneus subsp. tengcongensis | sn-glycerol 3-phosphate + choline | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | crystal structure | Caldanaerobacter subterraneus subsp. tengcongensis |
Synonyms | Comment | Organism |
---|---|---|
glycerophosphodiester phosphodiesterase | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
ttGDPD | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Caldanaerobacter subterraneus subsp. tengcongensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Caldanaerobacter subterraneus subsp. tengcongensis |