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Literature summary for 3.1.4.46 extracted from

  • Van Der Rest, B.; Rolland, N.; Boisson, A.M.; Ferro, M.; Bligny, R.; Douce, R.
    Identification and characterisation of plant glycerophosphodiester phosphodiesterase (2004), Biochem. J., 379, 601-607.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.46

Cloned(Commentary)

Cloned (Comment) Organism
-
 Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 40% inhibition at 5 mM Daucus carota
EDTA 40% inhibition at 5 mM Daucus carota
Fe2+ 90% inhibition at 0.1 mM Daucus carota
Zn2+ 40% inhibition at 5 mM Daucus carota

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
 glycerophosphocholine 30°C, pH 6.0 Daucus carota

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Daucus carota 5618
-
vacuole
-
 Daucus carota 5773
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, deduced from gene sequence Arabidopsis thaliana
55000
-
 x * 55000, SDS-PAGE Daucus carota
280000
-
 gel filtration Daucus carota

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9C907
-
-
Daucus carota
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Daucus carota

Purification (Commentary)

Purification (Comment) Organism
partial Daucus carota

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Daucus carota
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bis(glycerophospho)glycerol + H2O
-
 Daucus carota sn-gycerol 3-phosphate + ?
-
 ?
glycerophosphocholine + H2O
-
 Daucus carota sn-glycerol 3-phosphate + choline
-
 ?
glycerophosphoethanolamine + H2O
-
 Daucus carota sn-glycerol 3-phosphate + ethanolamine
-
 ?
glycerophosphoinositol + H2O
-
 Daucus carota ?
-
 ?
glycerophosphoserine + H2O
-
 Daucus carota ?
-
 ?
additional information no substrates are bis-p-nitrophenylphosphate, ADP, ADP-ribose, ADP-glucose, UDP-glucose Daucus carota ?
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Daucus carota
? x * 45000, deduced from gene sequence Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Daucus carota

pH Range

pH Minimum pH Maximum Comment Organism
4 10
-
 Daucus carota