Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) cells | Clostridium perfringens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, in 2.0 M (NH4)2SO4 with 3% (v/v) glycerol | Clostridium perfringens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-acetamido-1,2-dideoxynojirimycin | competitive inhibitor | Clostridium perfringens | |
6-acetamido-6-deoxycastanospermine | competitive inhibitor | Clostridium perfringens | |
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate | competitive inhibitor | Clostridium perfringens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
4-nitrophenyl alpha-N-acetyl-D-glucosaminide | in 0.1 M sodium phosphate, pH 7.3, at 30°C | Clostridium perfringens | |
0.74 | - |
dinitrophenyl alpha-N-acetyl-Dglucosaminide | in 0.1 M sodium phosphate, pH 7.3, at 30°C | Clostridium perfringens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Clostridium perfringens | 5764 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | - |
strain ATCC 13124 | - |
Purification (Comment) | Organism |
---|---|
- |
Clostridium perfringens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitrophenyl alpha-N-acetyl-D-glucosaminide + H2O | - |
Clostridium perfringens | 2,4-dinitrophenol + alpha-N-acetyl-D-glucosamine | - |
? | |
4-nitrophenyl alpha-N-acetyl-D-glucosaminide + H2O | - |
Clostridium perfringens | 4-nitrophenol + alpha-N-acetyl-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GH89 | - |
Clostridium perfringens |
lysosomal alpha-N-acetylglucosaminidase | - |
Clostridium perfringens |
NAGLU | - |
Clostridium perfringens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
4-nitrophenyl alpha-N-acetyl-D-glucosaminide | - |
Clostridium perfringens | |
8.5 | - |
dinitrophenyl alpha-N-acetyl-Dglucosaminide | - |
Clostridium perfringens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Clostridium perfringens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate | - |
Clostridium perfringens | |
0.92 | - |
6-acetamido-6-deoxycastanospermine | - |
Clostridium perfringens |