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Literature summary for 3.1.4.45 extracted from

  • Ficko-Blean, E.; Stubbs, K.A.; Nemirovsky, O.; Vocadlo, D.J.; Boraston, A.B.
    Structural and mechanistic insight into the basis of mucopolysaccharidosis IIIB (2008), Proc. Natl. Acad. Sci. USA, 105, 6560-6565.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) cells Clostridium perfringens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, in 2.0 M (NH4)2SO4 with 3% (v/v) glycerol Clostridium perfringens

Inhibitors

Inhibitors Comment Organism Structure
2-acetamido-1,2-dideoxynojirimycin competitive inhibitor Clostridium perfringens
6-acetamido-6-deoxycastanospermine competitive inhibitor Clostridium perfringens
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate competitive inhibitor Clostridium perfringens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
4-nitrophenyl alpha-N-acetyl-D-glucosaminide in 0.1 M sodium phosphate, pH 7.3, at 30°C Clostridium perfringens
0.74
-
dinitrophenyl alpha-N-acetyl-Dglucosaminide in 0.1 M sodium phosphate, pH 7.3, at 30°C Clostridium perfringens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
Clostridium perfringens 5764
-

Organism

Organism UniProt Comment Textmining
Clostridium perfringens
-
strain ATCC 13124
-

Purification (Commentary)

Purification (Comment) Organism
-
Clostridium perfringens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrophenyl alpha-N-acetyl-D-glucosaminide + H2O
-
Clostridium perfringens 2,4-dinitrophenol + alpha-N-acetyl-D-glucosamine
-
?
4-nitrophenyl alpha-N-acetyl-D-glucosaminide + H2O
-
Clostridium perfringens 4-nitrophenol + alpha-N-acetyl-D-glucosamine
-
?

Synonyms

Synonyms Comment Organism
GH89
-
Clostridium perfringens
lysosomal alpha-N-acetylglucosaminidase
-
Clostridium perfringens
NAGLU
-
Clostridium perfringens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.26
-
4-nitrophenyl alpha-N-acetyl-D-glucosaminide
-
Clostridium perfringens
8.5
-
dinitrophenyl alpha-N-acetyl-Dglucosaminide
-
Clostridium perfringens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
Clostridium perfringens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.062
-
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate
-
Clostridium perfringens
0.92
-
6-acetamido-6-deoxycastanospermine
-
Clostridium perfringens