Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.45 extracted from

  • Lee, W.S.; Rohrer, J.; Kornfeld, R.; Kornfeld, S.
    Multiple signals regulate trafficking of the mannose 6-phosphate-uncovering enzyme (2002), J. Biol. Chem., 277, 3544-3551.
    View publication on PubMed
Search for more literature for 3.1.4.45

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant UCE expression in mouse L-cells, expression of various green fluorescent protein-UCE fusion proteins in HeLa cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H510stop mutant with 7% of total enzyme activity on the cell surface at steady state compared with 1-3% of wild-type activity, kinetics of internalization Homo sapiens
M494stop mutant without increased cell surface expression Homo sapiens
Y486A mutated residue is required for efficient return of the enzyme from endosomes to the trans-Golgi network, mutant UCE is internalized normally but accumulates on the cell surface, 26-36% of activity on the cell surface compared with 1-3% of wild-type activity, because of increased recycling to the plasma membrane, kinetics of internalization Homo sapiens
Y486stop mutant with 16% of total enzyme activity on the cell surface at steady state compared with 1-3% of wild-type activity, kinetics of internalization Homo sapiens
Y488A mutant with 63% of total enzyme activity on the cell surface at steady state compared with 1-3% of wild-type activity, kinetics of internalization Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane with a transmembrane segment and a 41-residue cytoplasmic tail, resides primarily in the trans-Golgi network and cycles constitutively between the network and the plasma membrane, regulation of enzyme trafficking by multiple signals, e.g. the 486-YAYHPLQE-493 motif, which is recognized at several sorting sites and facilitates exit from the trans-Golgi network Homo sapiens 139
-
plasma membrane resides primarily in the trans-Golgi network and cycles between the network and the plasma membrane, UCE is endocytosed via clathrin-coated vesicles Homo sapiens 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal enzyme oligosaccharides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O
-
 Homo sapiens N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose
-
 ?
methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose + H2O
-
 Homo sapiens N-acetyl-D-glucosamine + methyl alpha-D-mannose 6-phosphate
-
 ?
additional information catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal enzyme oligosaccharides Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer
-
 Homo sapiens

Synonyms

Synonyms Comment Organism
mannose 6-phosphate-uncovering enzyme
-
 Homo sapiens
UCE uncovering enzyme Homo sapiens