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Literature summary for 3.1.4.45 extracted from

  • Do, H.; Lee, W.S.; Ghosh, P.; Hollowell, T.; Canfield, W.; Kornfeld, S.
    Human mannose 6-phosphate-uncovering enzyme is synthesized as a proenzyme that is activated by the endoprotease furin (2002), J. Biol. Chem., 277, 29737-29744.
    View publication on PubMed
Search for more literature for 3.1.4.45

Activating Compound

Activating Compound Comment Organism Structure
furin UCE is synthesized as an inactive proenzyme that is specifically activated by the endoprotease furin Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in mouse L-cells, in CHO-K1 cells and in Sf9 insect cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y488A mutation in the 488YHPL internalization signal present in the cytoplasmic tail, mutant UCE accumulates on the cell surface Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane mainly localized to the trans-Golgi network, cycles between the network and the plasma membrane, type I transmembrane glycoprotein, containing a 24-amino acid signal sequence, a luminal domain of 423 residues, a 27-residue transmembrane region and a 41-residue cytoplasmic tail Homo sapiens 139
-
plasma membrane mainly localized to the trans-Golgi network and cycles between the network and plasma membrane Homo sapiens 5886
-
soluble recombinant UCE expressed in CHO-K1 cells and in Sf9 insect cells Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O Homo sapiens removes a covering N-acetylglucosamine from the mannose 6-phosphate recognition marker on lysosomal acid hydrolases N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens
proteolytic modification UCE is synthesized as an inactive proenzyme that is activated by the endoprotease furin, which is localized in the trans-Golgi network and cleaves an RARLPR/D sequence to release a 24-amino acid propiece Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
499fold: recombinant UCE expressed in CHO-K1 cells, 1392fold: recombinant UCE expressed in Sf9 insect cells Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
L-cell mouse WT 1-38 L-cells expressing human wild-type UCE Homo sapiens
-
LoVo cell derived from a lymph node metastasis of a human colon adenocarcinoma, CCL-229, extremely low UCE activity due to a lack of the activating endoprotease furin, addition of furin restores UCE activity Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2
-
 recombinant UCE expressed in CHO-K1 cells Homo sapiens
4.75
-
 recombinant UCE expressed in Sf9 insect cells Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-O-(N-acetyl-alpha-D-glucosaminyl)phosphono-D-mannopyranose + H2O
-
 Homo sapiens N-acetyl-D-glucosamine + 6-O-phosphono-D-mannopyranose
-
 ?
glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O removes a covering N-acetylglucosamine from the mannose 6-phosphate recognition marker on lysosomal acid hydrolases Homo sapiens N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose
-
 ?
methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose + H2O
-
 Homo sapiens N-acetyl-D-glucosamine + methyl alpha-D-mannose 6-phosphate
-
 ?
UDP-N-acetyl-D-glucosamine + H2O
-
 Homo sapiens UDP + N-acetyl-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
tetramer composed of two disulfide-linked homodimers Homo sapiens

Synonyms

Synonyms Comment Organism
UCE uncovering enzyme Homo sapiens