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Literature summary for 3.1.4.45 extracted from
- Purification and kinetic parameters of bovine liver N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (1994), J. Biol. Chem., 269, 1718-1726.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CdSO4 | inhibits activity by 70% | Bos taurus |  |
| CdSO4 | - |
Homo sapiens | |
| dithiothreitol | - |
Bos taurus | |
| dithiothreitol | 10 mM 15 min at 37°C inhibits activity by 90% | Homo sapiens | |
| MgCl2 | inhibits activity by 30% | Bos taurus | |
| MnCl2 | inhibits activity by 30% | Bos taurus | |
| N-acetylglucosamine | - |
Bos taurus | |
| N-acetylglucosamine | - |
Homo sapiens | |
| N-acetylglucosamine phosphate | - |
Bos taurus | |
| N-acetylglucosamine phosphate | - |
Homo sapiens | |
| PO43- | - |
Bos taurus | |
| PO43- | - |
Homo sapiens | |
| SDS | 0.02% SDS inhibits activity by 14% | Bos taurus | |
| UDP-GlcNAc | - |
Bos taurus | |
| UDP-GlcNAc | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.734 | - |
UDP-GlcNAc | - |
Bos taurus | |
| 0.74 | - |
methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
- |
Homo sapiens |
| 69000 | - |
fetal calf serum, reducing SDS-PAGE | Bos taurus |
| 121000 | - |
liver, nonreducing SDS-PAGE | Bos taurus |
| 129000 | - |
liver, nonreducing SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, superose 6 eluate, 3 months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose + H2O | - |
Homo sapiens | N-acetyl-D-glucosamine + methyl alpha-D-mannose 6-phosphate | - |
? | |
| methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose + H2O | - |
Bos taurus | N-acetyl-D-glucosamine + methyl alpha-D-mannose 6-phosphate | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + H2O | - |
Homo sapiens | N-acetyl-D-glucosamine + UDP | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + H2O | - |
Bos taurus | N-acetyl-D-glucosamine + UDP | - |
? | |
| UDP-N-acetyl-D-glucosamine + H2O | - |
Bos taurus | UDP + N-acetyl-D-glucosamine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
after 3 min 80% of activity | Bos taurus |
| 75 | - |
after 3 min only 7.6% activity | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | - |
- |
Homo sapiens |
| 6.7 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | liver | Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | at pH 4, 54% of activity, at pH 10, 83% of activity | Bos taurus |
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