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Literature summary for 3.1.4.45 extracted from
- Purification and characterization of human lymphoblast N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (1996), Glycobiology, 6, 619-626.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetoamino-deoxycastanospermine | - |
Homo sapiens |  |
| Cu2+ | reduces activity by 30-40% | Homo sapiens | |
| Hg2+ | reduces activity by 30-40% | Homo sapiens | |
| N-acetylglucosamine | - |
Homo sapiens | |
| N-acetylglucosamine phosphate | - |
Homo sapiens | |
| Zn2+ | inhibits by 50% | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
N-[6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannosyl]-[uteroferrin]-L-asparagine | - |
Homo sapiens | |
| 0.76 | - |
methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose | - |
Homo sapiens | |
| 0.78 | - |
UDP-GlcNAc | - |
Homo sapiens | |
| 31 | - |
N-acetylglucosamine-phosphate | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 114000 | - |
lymphoblasts, nonreducing SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| lymphoblast | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 1 month | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose + H2O | - |
Homo sapiens | N-acetyl-D-glucosamine + methyl alpha-D-mannose 6-phosphate | - |
? | |
| N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Homo sapiens | N-acetyl-D-glucosamine + phosphate | - |
? | |
| N-[6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannosyl]-[uteroferrin]-L-asparagine + H2O | - |
Homo sapiens | N-acetyl-D-glucosamine + N-[6-phospho-alpha-D-mannosyl]-[uteroferrin]-L-asparagine | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + H2O | - |
Homo sapiens | N-acetyl-D-glucosamine + UDP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable for 10 min | Homo sapiens |
| 70 | - |
activity diminished to 20% and 10% | Homo sapiens |
| 80 | - |
activity diminished to 20% and 10% | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Homo sapiens |
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