Literature summary for 3.1.4.44 extracted from

Gallazzini, M.; Ferraris, J.D.; Burg, M.B.
GDPD5 is a glycerophosphocholine phosphodiesterase that osmotically regulates the osmoprotective organic osmolyte GPC (2008), Proc. Natl. Acad. Sci. USA, 105, 11026-11031.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in mIMCD-3 cell: GDPD5 expression reduces (mRNA level) when osmolarity is increased from 300 to 700 mosmol/kg by adding NaCl (200 mosmol/kg) and urea (200 mosmol/kg) in combination. HEK293 cells transfected with GDPD5: recombinant GDPD5 is perinuclear. GDPD5 colocalizes with neuropathy target esterase in the ER of HEK293 cells. Expression of GDPD5 increases GPC-PDE activity (specific siRNA assay to knock down GDPD5, mIMCD-3 cells)	Mus musculus

Cloned (Comment)

Organism

expression in mIMCD-3 cell: GDPD5 expression reduces (mRNA level) when osmolarity is increased from 300 to 700 mosmol/kg by adding NaCl (200 mosmol/kg) and urea (200 mosmol/kg) in combination. HEK293 cells transfected with GDPD5: recombinant GDPD5 is perinuclear. GDPD5 colocalizes with neuropathy target esterase in the ER of HEK293 cells. Expression of GDPD5 increases GPC-PDE activity (specific siRNA assay to knock down GDPD5, mIMCD-3 cells)

Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
Urea	in vivo: activity decreases in vivo (mIMCD-3 cell), measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine. in vitro: no directl inhibition of GDPD5 activity, GPC-PDE in vitro activity is reduced by over 60% in immuno-precipitates from HEK293 cells exposed to high urea concentration.	Mus musculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
KCl	in vitro: no direct decrease in GDPD5 activity, measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine	Mus musculus
NaCl	in vivo: activity decreases (mIMCD-3 cell), measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine. in vitro: no direct decrease in GDPD5 activity, GPC-PDE in vitro activity is reduced by over 60% in immuno-precipitates from HEK293 cells exposed to high NaCl concentration.	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q640M6	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
IMCD-3 cell	-	Mus musculus	-

Synonyms

Synonyms	Comment	Organism
glycerophosphodiester phosphodiesterase domain containing 5	GDPD5, BC024955	Mus musculus