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Literature summary for 3.1.4.44 extracted from
- GDE1/MIR16 is a glycerophosphoinositol phosphodiesterase regulated by stimulation of G protein-coupled receptors (2003), Proc. Natl. Acad. Sci. USA, 100, 1745-1750.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | GDE1 activity expressed in HEK 293T cells can be regulated by stimulation of G protein-coupled, alpha/beta-adrenergic and lysophospholipid receptors, isoproterenol increases the GDE1 activity by 50% compared with untreated cells | Rattus norvegicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in HEK 293T cells | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E97A/D99A | catalytic domain double mutant with negligible activity | Rattus norvegicus |
| H112A | catalytic domain mutant with negligible activity | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glycerophosphoethanolamine | blocks the hydrolysis of glycerophosphoinositol by 30% | Rattus norvegicus |  |
| glycerophosphoinositol 4,5-diphosphate | blocks the hydrolysis of glycerophosphoinositol by 70% | Rattus norvegicus | |
| Glycerophosphoinositol 4-phosphate | blocks the hydrolysis of glycerophosphoinositol by 30% | Rattus norvegicus | |
| glycerophosphoserine | blocks the hydrolysis of glycerophosphoinositol by 80% | Rattus norvegicus | |
| additional information | GDE1 activity expressed in HEK 293T cells can be regulated by stimulation of G protein-coupled, alpha/beta-adrenergic and lysophospholipid receptors, phenylephrine reduces the GDE1 activity by 30% and lysophatidic acid by 40% compared with untreated cells | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Rattus norvegicus | |
| 12 | - |
1-(sn-glycero-3-phospho)-1D-myo-inositol | pH 7.5, 37°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | integral membrane protein, the catalytic GDE domain faces the lumen/extracellular space and the C-terminus faces the cytoplasm | Rattus norvegicus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 10 mM, 3fold activation | Rattus norvegicus | |
| additional information | not activated by Ca2+ | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | regulation of GDE1 activity by G protein signalling, GDE1 provides a link between phosphoinositide metabolism and G protein signal transduction, GDE1 is most likely involved in the remodeling of membrane phosphoinositides and regulation of membrane phospholipid composition, and hence in intracellular membrane trafficking | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-(sn-glycero-3-phospho)-1D-myo-inositol + H2O | selectively hydrolyzes glycerophosphoinositol over glycerophosphocholine | Rattus norvegicus | myo-inositol + sn-glycerol 3-phosphate | - |
? | |
| glycerophosphocholine + H2O | selectively hydrolyzes glycerophosphoinositol over glycerophosphocholine | Rattus norvegicus | choline + glycerolphosphate | - |
? | |
| additional information | glycerophosphoinositol 4-phosphate and glycerophosphoinositol 4,5-diphosphate are potential substrates | Rattus norvegicus | ? | - |
? | |
| additional information | regulation of GDE1 activity by G protein signalling, GDE1 provides a link between phosphoinositide metabolism and G protein signal transduction, GDE1 is most likely involved in the remodeling of membrane phosphoinositides and regulation of membrane phospholipid composition, and hence in intracellular membrane trafficking | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDE1 | formerly MIR16, membrane interacting protein of RGS16 | Rattus norvegicus |
| GPI-PDE | - |
Rattus norvegicus |
| sn-glycero-3-phospho-1-inositol glycerophosphohydrolase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
around | Rattus norvegicus |
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Release 2023.1 (January 2023)
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