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Literature summary for 3.1.4.41 extracted from
- Bacterial and arachnid sphingomyelinases D comparison of biophysical and pathological activities (2017), J. Cell. Biochem., 118, 2053-2063 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)RIL cells | Corynebacterium pseudotuberculosis |
| expressed in Escherichia coli BL21(DE3)RIL cells | Loxosceles intermedia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00076 | - |
sphingomyelin | at pH 7.4 and 37°C | Loxosceles intermedia |  |
| 0.00082 | - |
lysophosphatidylcholine | at pH 7.4 and 37°C | Loxosceles intermedia | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sphingomyelin + H2O | Corynebacterium pseudotuberculosis | - |
ceramide 1-phosphate + choline | - |
? | |
| sphingomyelin + H2O | Loxosceles intermedia | - |
ceramide 1-phosphate + choline | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium pseudotuberculosis | - |
- |
- |
| Loxosceles intermedia | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, Mono S column chromatography, and Superdex 75 gel filtration | Corynebacterium pseudotuberculosis |
| Ni-NTA column chromatography, Mono S column chromatography, and Superdex 75 gel filtration | Loxosceles intermedia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lysophosphatidylcholine + H2O | - |
Corynebacterium pseudotuberculosis | lysophosphatidic acid + choline | - |
? | |
| lysophosphatidylcholine + H2O | - |
Loxosceles intermedia | lysophosphatidic acid + choline | - |
? | |
| sphingomyelin + H2O | - |
Corynebacterium pseudotuberculosis | ceramide 1-phosphate + choline | - |
? | |
| sphingomyelin + H2O | - |
Loxosceles intermedia | ceramide 1-phosphate + choline | - |
? | |
| sphingomyelin + H2O | the enzyme shows a marked preference for sphingomyelin over lysophosphatidylcholine | Corynebacterium pseudotuberculosis | ceramide 1-phosphate + choline | - |
? | |
| sphingomyelin + H2O | the enzyme shows a marked preference for sphingomyelin over lysophosphatidylcholine | Loxosceles intermedia | ceramide 1-phosphate + choline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, SDS-PAGE | Corynebacterium pseudotuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RecDT1 | - |
Loxosceles intermedia |
| SMase D | - |
Corynebacterium pseudotuberculosis |
| SMase D | - |
Loxosceles intermedia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Corynebacterium pseudotuberculosis |
| 7.4 | - |
- |
Loxosceles intermedia |
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Release 2023.1 (January 2023)
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