Literature summary for 3.1.4.41 extracted from

Kusma, J.; Chaim, O.M.; Wille, A.C.; Ferrer, V.P.; Sade, Y.B.; Donatti, L.; Gremski, W.; Mangili, O.C.; Veiga, S.S.
Nephrotoxicity caused by brown spider venom phospholipase-D (dermonecrotic toxin) depends on catalytic activity (2008), Biochimie, 90, 1722-1736.

Search for more literature for 3.1.4.41

Application

Application	Comment	Organism
medicine	a molecular mechanism for Loxosceles venom nephrotoxicity is demonstrated that is dependent on the catalytic activity of phospholipase-D toxin	Loxosceles intermedia

Cloned(Commentary)

Cloned (Comment)	Organism
expressed as a His-tagged fusion protein	Loxosceles intermedia

Protein Variants

Protein Variants	Comment	Organism
H12A	mice are exposed to recombinant wild-type phospholipase-D, or to an isoform with a H12A mutation in the catalytic domain resulting in no phospholipasic activity. Renal biopsies from mice treated with the wild-type toxin show glomerular edema, erythrocytes and collapse of Bowmans space, edema and deposition of proteinaceous material within the tubular lumen. An absence of nephrotoxicity is shown in mice treated with the mutated toxin. Analyses of urine and blood show that wild type toxin induces hematuria and elevation of blood urea, while treatment with mutated toxin causes no changes. Mouse lethality experiments also show oliguria and mortality after treatment with wild-type toxin, but not following exposure to the mutated toxin. Wild-type toxin treatment causes cytoplasmic vacuolization, impaired spreading, reduction of cellular viability, and cell-cell and cell-substratum detachment in MDCK cells, while treatment with mutated isoform has no effect	Loxosceles intermedia

Organism

Organism	UniProt	Comment	Textmining
Loxosceles intermedia	P0CE81	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Loxosceles intermedia

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sphingomyelin + H2O	-	Loxosceles intermedia	ceramide-1-phosphate + choline	-	?

Synonyms

Synonyms	Comment	Organism
phospholipase D	-	Loxosceles intermedia
PLD	-	Loxosceles intermedia

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Loxosceles intermedia

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Release 2023.1 (January 2023)