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Literature summary for 3.1.4.4 extracted from
- Improving phospholipase D activity and selectivity by bio-imprinting-immobilization to produce phosphatidylglycerol (2018), J. Biotechnol., 281, 67-73 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | hyperactivation of PLD by bio-imprinting and immobilization by adsorption and precipitation, followed by cross-linking results in increased catalytic activity. The maximum activity of immobilized PLD reaches 166953 U/g protein, compared to 11922 U/mg protein for the free form. The selectivity of PLD is significantly enhanced after immobilization. The yield of phosphatidylglycerol and phosphatidic acid reaches 94.0% and 5.96%, respectively | Streptomyces sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces sp. | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Streptomyces sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.92 | - |
free enzyme, pH 5.5, temperature not specified in the publication | Streptomyces sp. |
| 166.9 | - |
bio-imprinted immobilized enzyme, pH 6.0, temperature not specified in the publication | Streptomyces sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | - |
Streptomyces sp. | choline + phosphatidate | - |
? |  |
| phosphatidylethanolamine + H2O | - |
Streptomyces sp. | ethanolamine + phosphatidate | - |
? | |
| phosphatidylglycerol + H2O | - |
Streptomyces sp. | glycerol + phosphatidate | - |
? | |
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Release 2023.1 (January 2023)
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