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Literature summary for 3.1.4.4 extracted from
- Identification of a novel phospholipase D with high transphosphatidylation activity and its application in synthesis of phosphatidylserine and DHA-phosphatidylserine (2017), J. Biotechnol., 249, 51-58 .
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| homology modeling of structure and docking of substrates | Acinetobacter radioresistens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 10 mM, 91% of initial activity | Acinetobacter radioresistens |  |
| Fe3+ | 10 mM, complete loss of activity | Acinetobacter radioresistens | |
| Mg2+ | 10 mM, 78% of initial activity | Acinetobacter radioresistens | |
| Zn2+ | 10 mM, 47% of initial activity | Acinetobacter radioresistens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, 255% of initial activity | Acinetobacter radioresistens | |
| K+ | 10 mM, 153% of initial activity | Acinetobacter radioresistens | |
| Na+ | 10 mM, 142% of initial activity | Acinetobacter radioresistens | |
| Ni2+ | 10 mM, 184% of initial activity | Acinetobacter radioresistens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter radioresistens | - |
- |
- |
| Acinetobacter radioresistens a2 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| docosahexanoylphosphatidylcholine + D-serine | - |
Acinetobacter radioresistens | docosahexanoylphosphatidylserine + choline | - |
? | |
| docosahexanoylphosphatidylcholine + D-serine | - |
Acinetobacter radioresistens a2 | docosahexanoylphosphatidylserine + choline | - |
? | |
| additional information | enzyme displays high transphosphatidylation activity and no hydrolysis activity. In the PLD-catalyzed synthesis process (12 h), both the transphosphatidylation conversion rate and selectivity of phosphatidylserine and docosahexaenoic acid-phosphatidylserine are about 100% | Acinetobacter radioresistens | ? | - |
? | |
| additional information | enzyme displays high transphosphatidylation activity and no hydrolysis activity. In the PLD-catalyzed synthesis process (12 h), both the transphosphatidylation conversion rate and selectivity of phosphatidylserine and docosahexaenoic acid-phosphatidylserine are about 100% | Acinetobacter radioresistens a2 | ? | - |
? | |
| phosphatidylcholine + D-serine | - |
Acinetobacter radioresistens | phosphatidylserine + choline | - |
? | |
| phosphatidylcholine + D-serine | - |
Acinetobacter radioresistens a2 | phosphatidylserine + choline | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
maximum transphosphatidylation activity | Acinetobacter radioresistens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | more than 80% of maximum transphosphatidylation activity | Acinetobacter radioresistens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
maximum transphosphatidylation activity | Acinetobacter radioresistens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | more than 60% of maximum transphosphatidylation activity | Acinetobacter radioresistens |
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