Any feedback?
Literature summary for 3.1.4.4 extracted from
- mu-opioid receptor-stimulated synthesis of reactive oxygen species is mediated via phospholipase D2 (2009), J. Neurochem., 110, 1288-1296.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| beta-endorphin | opioid receptor-mediated activation | Homo sapiens |  |
| additional information | [D-Ala2,Me Phe4,Glyol5]enkephalin and beta-endorphin strongly induce PLD2 activation, whereas the non-endocytotic drugs morphine and buprenorphine do not activate PLD2 | Homo sapiens | |
| phorbol-12-myristate-13-acetate | induces PLD2 activation | Homo sapiens | |
| [D-Ala2,Me Phe4,Glyol5]enkephalin | opioid receptor-mediated activation | Homo sapiens | |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | siRNA-mediated reduction of endogenous PLD2 expression results in a nearly complete inhibition of the opioid-induced H2O2 production in Rattus norvegicus mu-opioid receptor MOPr-expressing HEK293 cells, while in control cells it has no effect on [D-Ala2,Me Phe4,Glyol5]enkephalin and beta-endorphin effects on the DCFDA fluorescence | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| sphinganine | does not alter basal PLD2 activity, but inhibits PLD2 activation induced by [D-Ala2,Me Phe4,Glyol5]enkephalin and beta-endorphin, overview | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | Homo sapiens | - |
choline + phosphatidic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
isozyme PLD2 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HEK-293 cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme also performs transphosphatidylation transferring phosphatidic acid to a primary alcohol | Homo sapiens | ? | - |
? | |
| phosphatidylcholine + H2O | - |
Homo sapiens | choline + phosphatidic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phospholipase D2 | - |
Homo sapiens |
| PLD2 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | essential role of PLD2 activity in the opioid-mediated induction of reactive oxygen species synthesis | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
