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Literature summary for 3.1.4.4 extracted from
- Phospholipase D from Allium sativum bulbs: A highly active and thermal stable enzyme (2008), Int. J. Biol. Macromol., 42, 380-385.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidylinositol 4,5-bisphosphate | - |
Allium sativum |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | maximal activity at 70 mM Ca2+ concentration. However, Ca2+ is not mandatory for enzymatic activity | Allium sativum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Allium sativum | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bulb | - |
Allium sativum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + ethanol | enzyme shows also transphosphatidylation activity | Allium sativum | phosphatidylethanol + choline | - |
? | |
| phosphatidylcholine + H2O | - |
Allium sativum | phosphatidate + choline | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phospholipase D | - |
Allium sativum |
| PLDGB | - |
Allium sativum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Allium sativum |
| 70 | - |
maximal activity | Allium sativum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | enzyme shows more than 65% of maximal activity | Allium sativum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
the crude enzyme retains almost 100% activity after incubation for 30 min at 60°C, after which the activity increases and is about 119% and 139% after incubation for 30 min at 70 and 80°C, respectively | Allium sativum |
| 80 | - |
enzyme shows 90% activity | Allium sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Allium sativum |
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