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Literature summary for 3.1.4.4 extracted from

  • Simkhada, J.R.; Lee, H.J.; Jang, S.Y.; Cho, S.S.; Park, E.J.; Sohng, J.K.; Yoo, J.C.
    A novel alkalo- and thermostable phospholipase D from Streptomyces olivochromogenes (2009), Biotechnol. Lett., 31, 429-435.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
CHAPS relative activity: 201 Streptomyces olivochromogenes
deoxycholic acid relative activity: 246% Streptomyces olivochromogenes
polyethylene-4-laurylether relative activity: 125% Streptomyces olivochromogenes
SDS relative activity: 189 Streptomyces olivochromogenes
Triton X-100 relative activity: 429% Streptomyces olivochromogenes
Tween-20 relative activity: 257% Streptomyces olivochromogenes
Tween-80 relative activity: 249% Streptomyces olivochromogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
phosphatidylcholine Vmax: 650 micromol/min/mg Streptomyces olivochromogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ enzyme activity is Ca2+-dependent Streptomyces olivochromogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
SDS-PAGE Streptomyces olivochromogenes

Organism

Organism UniProt Comment Textmining
Streptomyces olivochromogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a one-step chromatography on Sepharose CL-6B colum Streptomyces olivochromogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + glycerol
-
Streptomyces olivochromogenes phosphatidylglycerol + choline
-
?
phosphatidylcholine + H2O
-
Streptomyces olivochromogenes 1,2-diacylglycerophosphate + choline
-
?
phosphatidylcholine + L-serine
-
Streptomyces olivochromogenes phosphatidylserine + choline
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Streptomyces olivochromogenes

Synonyms

Synonyms Comment Organism
phospholipase D
-
Streptomyces olivochromogenes
PLD
-
Streptomyces olivochromogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
transphosphatidylation activity highest at Streptomyces olivochromogenes
75
-
maximal hydrolytic activity Streptomyces olivochromogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 75
-
Streptomyces olivochromogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
hydrolytic and transphosphatidylation activity highest at Streptomyces olivochromogenes

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 13
-
Streptomyces olivochromogenes