Application | Comment | Organism |
---|---|---|
synthesis | heterologous high-yield expression of enzyme in Pichia pastoris and purification by single calcium-dependent octyl-sepharose chromatography step gives 8 mg of pure recombinant protein from 1 l of yeast culture without proteolytic degradation of the N-terminal calcium-dependent C2 lipid binding domain | Vigna unguiculata |
Cloned (Comment) | Organism |
---|---|
high-yield expression in Pichia pastoris | Vigna unguiculata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88110 | - |
x * 90000, SDS-PAGE, x * 88110, mass spectrometry | Vigna unguiculata |
90000 | - |
x * 90000, SDS-PAGE, x * 88110, mass spectrometry | Vigna unguiculata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna unguiculata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification by single calcium-dependent octyl-sepharose chromatography step | Vigna unguiculata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27 | - |
pH 8.0, 37°C | Vigna unguiculata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | - |
Vigna unguiculata | 1,2-diacylglycerophosphate + choline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, SDS-PAGE, x * 88110, mass spectrometry | Vigna unguiculata |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Vigna unguiculata | isoelectric focusing | - |
4.7 |