Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
367stop | complete loss of activity | Rattus norvegicus |
377stop | complete loss of activity | Rattus norvegicus |
387stop | activity comparable to wild-type | Rattus norvegicus |
C170S | activity comparable to wild-type | Rattus norvegicus |
C222S | activity comparable to wild-type | Rattus norvegicus |
C224S | considerable reduction in activity | Rattus norvegicus |
C237S | activity comparable to wild-type | Rattus norvegicus |
C255S | activity comparable to wild-type | Rattus norvegicus |
C288S | activity comparable to wild-type | Rattus norvegicus |
D147N | complete loss of activity | Rattus norvegicus |
D189N | less than 0.1% of wild-type activity | Rattus norvegicus |
D284N | complete loss of activity | Rattus norvegicus |
D389N | single nucleotide polymorphism, activity comparable to wild-type | Homo sapiens |
DELTAN138 | complete loss of activity | Rattus norvegicus |
DELTAN55 | activity comparable to wild-type | Rattus norvegicus |
DELTAN85 | complete loss of activity | Rattus norvegicus |
H185N | less than 0.1% of wild-type activity | Rattus norvegicus |
H187N | less than 0.1% of wild-type activity | Rattus norvegicus |
H190N | less than 0.1% of wild-type activity | Rattus norvegicus |
H253N | complete loss of activity | Rattus norvegicus |
H321N | complete loss of activity | Rattus norvegicus |
H331N | 4% of wild-type activity | Rattus norvegicus |
H343N | activity comparable to wild-type | Rattus norvegicus |
H353N | activity comparable to wild-type | Rattus norvegicus |
H380R | single nucleotide polymorphism, complete loss of activity | Homo sapiens |
H380R | single nucleotide polymorphism, low catalytic activity | Rattus norvegicus |
L207F | single nucleotide polymorphism, complete loss of activity | Homo sapiens |
L207F | single nucleotide polymorphism, low catalytic activity | Rattus norvegicus |
S152A | single nucleotide polymorphism, activity comparable to wild-type | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0029 | - |
N-palmitoyl-phosphatidylethanolamine | mutant D389N, 37°C, pH 7.4, presence of 10 mM MgCl2 | Homo sapiens | |
0.0029 | - |
N-palmitoyl-phosphatidylethanolamine | mutant DELTAN55, 37°C, pH 7.4, presence of 10 mM MgCl2 | Rattus norvegicus | |
0.0036 | - |
N-palmitoyl-phosphatidylethanolamine | mutant 387stop, 37°C, pH 7.4, presence of 10 mM MgCl2 | Rattus norvegicus | |
0.0037 | - |
N-palmitoyl-phosphatidylethanolamine | mutant S152A, 37°C, pH 7.4, presence of 10 mM MgCl2 | Homo sapiens | |
0.0042 | - |
N-palmitoyl-phosphatidylethanolamine | mutant C237S, 37°C, pH 7.4, presence of 10 mM MgCl2 | Rattus norvegicus | |
0.0043 | - |
N-palmitoyl-phosphatidylethanolamine | mutant C222S, 37°C, pH 7.4, presence of 10 mM MgCl2 | Rattus norvegicus | |
0.0045 | - |
N-palmitoyl-phosphatidylethanolamine | mutant C288S, 37°C, pH 7.4, presence of 10 mM MgCl2 | Rattus norvegicus | |
0.0046 | - |
N-palmitoyl-phosphatidylethanolamine | wild-type, 37°C, pH 7.4, presence of 10 mM MgCl2 | Homo sapiens | |
0.0047 | - |
N-palmitoyl-phosphatidylethanolamine | mutant C170S, 37°C, pH 7.4, presence of 10 mM MgCl2 | Rattus norvegicus | |
0.0047 | - |
N-palmitoyl-phosphatidylethanolamine | wild-type, 37°C, pH 7.4, presence of 10 mM MgCl2 | Rattus norvegicus | |
0.0059 | - |
N-palmitoyl-phosphatidylethanolamine | wild-type, 37°C, pH 7.4 | Rattus norvegicus | |
0.0061 | - |
N-palmitoyl-phosphatidylethanolamine | mutant C255S, 37°C, pH 7.4, presence of 10 mM MgCl2 | Rattus norvegicus | |
0.0143 | - |
N-palmitoyl-phosphatidylethanolamine | mutant C224S, 37°C, pH 7.4, presence of 10 mM MgCl2 | Rattus norvegicus | |
0.0211 | - |
N-palmitoyl-phosphatidylethanolamine | mutant H331N, 37°C, pH 7.4, presence of 10 mM MgCl2 | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 50% activation at 2.3 mM | Rattus norvegicus | |
Mg2+ | 50% activation at 2.3 mM | Rattus norvegicus | |
Zn2+ | catalytically active Zn2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
N-acylethanolamine-specific phospholipase D, NAPE-PLD | - |
Rattus norvegicus | - |
N-acylethanolamine-specific phospholipase D, NAPE-PLD | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.016 | - |
37°C, pH 7.4, presence of 10 mM MgCl2, substrate N-palmitoyl-phosphatidylethanolamine | Rattus norvegicus |
2.4 | - |
37°C, pH 7.4, presence of 10 mM MgCl2, substrate N-lauroyl-phosphatidylethanolamine | Rattus norvegicus |
Storage Stability | Organism |
---|---|
unstable, one cycle of freezeing and thawing causes loss of up to 50% of enzymic activity. Addition of 1% w/v octyl glucoside improves stability | Homo sapiens |
unstable, one cycle of freezing and thawing causes loss of up to 50% of enzymic activity. Addition of 1% w/v octyl glucoside improves stability | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerophospho-(N-palmitoyl)ethanolamine + H2O | - |
Rattus norvegicus | N-palmitoylethanolamine + ? | 1% of the activity with N-palmitoyl-phosphatidylethanolamine | ? | |
additional information | no substrate: N-palmitoylethanolamine phosphate, phosphatidylcholine, phosphatidylserine, phosphatidylinositol | Rattus norvegicus | ? | - |
? | |
N-acyl-phosphatidylethanolamine + H2O | high specificity for N-acyl-phosphatidylethanolamines without selectivity for long chain or medium chain N-acyl species | Homo sapiens | N-acylethanolamine + phosphatidate | - |
? | |
N-acyl-phosphatidylethanolamine + H2O | high specificity for N-acyl-phosphatidylethanolamines without selectivity for long chain or medium chain N-acyl species | Rattus norvegicus | N-acylethanolamine + phosphatidate | - |
? | |
N-lauroyl-phosphatidylethanolamine + H2O | - |
Homo sapiens | phosphatidic acid + N-lauroylethanolamine | - |
? | |
N-lauroyl-phosphatidylethanolamine + H2O | - |
Rattus norvegicus | phosphatidic acid + N-lauroylethanolamine | - |
? | |
N-palmitoyl-lyso-phosphatidylethanolamine + H2O | - |
Rattus norvegicus | N-palmitoylethanolamine + sn-glycerol 3-phosphate | 4% of the activity with N-palmitoyl-phosphatidylethanolamine | ? | |
N-palmitoyl-phosphatidylethanolamine + H2O | - |
Homo sapiens | phosphatidic acid + N-palmitoylethanolamine | - |
? | |
N-palmitoyl-phosphatidylethanolamine + H2O | - |
Rattus norvegicus | phosphatidic acid + N-palmitoylethanolamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
NAPE-PLD | - |
Rattus norvegicus |