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Literature summary for 3.1.4.4 extracted from

  • Tsuruta, H.; Hayashi, R.; Ohkawa, H.; Ohkatsu, N.; Morimoto, M.; Nishimoto, K.; Santou, S.; Aizono, Y.
    Characterisitics and gene cloning of phospholipase D of the psychrophile, Shewanella sp. (2007), Biosci. Biotechnol. Biochem., 71, 2534-2542.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Shewanella sp.

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation Shewanella sp.
Co2+ 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation Shewanella sp.
Cu2+ 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation Shewanella sp.
Fe3+ 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation Shewanella sp.
Mg2+ 2.5 mM, inhibitory to both hydrolytic activity and transphosphatidylation Shewanella sp.
Mn2+ 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation Shewanella sp.
Ni2+ 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation Shewanella sp.
Zn2+ 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation Shewanella sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ presence of Ca2+ enhances 1.6fold hydrolytic activiy and 6fold transphosphatidylation Shewanella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
x * 64000, SDS-PAGE Shewanella sp.

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol enzyme shows high activity to the non-micelle form of phosphatidylcholine in an aequeous solution containing methanol, ethanol, iso-propanol, or n-propanol Shewanella sp.
isopropanol enzyme shows high activity to the non-micelle form of phosphatidylcholine in an aequeous solution containing methanol, ethanol, isopropanol, or n-propanol Shewanella sp.
Methanol assay in presence of 50% ethanol Shewanella sp.
n-propanol enzyme shows high activity to the non-micelle form of phosphatidylcholine in an aequeous solution containing methanol, ethanol, isopropanol, or n-propanol Shewanella sp.

Organism

Organism UniProt Comment Textmining
Shewanella sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Shewanella sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.076
-
pH 7.8, 25°C, presence of 50% methanol Shewanella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme exhibits a ratio of hydrolytic activity to transphosphatidylation of 2.5 Shewanella sp. ?
-
?
phosphatidylcholine + H2O high activity to the non-micelle form of phosphatidylcholine in an aequeous solution containing methanol, ethanol, isopropanol, or n-propanol. In absence of alcohol, hydrolytic activity is weak, and no transphosphatidylation activity is detected Shewanella sp. 1,2-diacylglycerophosphate + choline
-
?

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE Shewanella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
presence of Ca2+ Shewanella sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10
-
6.5% of maximum activity Shewanella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
stable below Shewanella sp.
40
-
rather labile above Shewanella sp.
60
-
rapid loss of activity Shewanella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Shewanella sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 enzyme is labile in the low pH range Shewanella sp.