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Literature summary for 3.1.4.4 extracted from
- Identification, cloning and functional characterization of a novel dermonecrotic toxin (phospholipase D) from brown spider (Loxosceles intermedia) venom (2008), Biochim. Biophys. Acta, 1780, 167-178.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Loxosceles intermedia |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32300 | - |
x * 32300, calculated, x * 33000, SDS-PAGE, recombinant enzyme | Loxosceles intermedia |
| 33000 | - |
x * 32300, calculated, x * 33000, SDS-PAGE, recombinant enzyme | Loxosceles intermedia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Loxosceles intermedia | enzyme shows dermonbecrotic properties. Enzyme causes massive inflammatory response in rabbit skin dermis, evokes platelet aggregation, increases vascular permeability, causes edema and death in mice | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Loxosceles intermedia | A4USB4 | isoform 6 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Loxosceles intermedia | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme shows dermonbecrotic properties. Enzyme causes massive inflammatory response in rabbit skin dermis, evokes platelet aggregation, increases vascular permeability, causes edema and death in mice | Loxosceles intermedia | ? | - |
? | |
| sphingomyelin + H2O | - |
Loxosceles intermedia | N-acylsphingosylphosphate + choline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32300, calculated, x * 33000, SDS-PAGE, recombinant enzyme | Loxosceles intermedia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dermonecrotic toxin | - |
Loxosceles intermedia |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Loxosceles intermedia | calculated | - |
8.2 |
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