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Literature summary for 3.1.4.4 extracted from
- Evidence for and characterization of Ca2+ binding to the catalytic region of Arabidopsis thaliana phospholipase Dbeta (2004), J. Biol. Chem., 279, 47833-47839.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidylinositol 4,5-bisphosphate | the interaction of Ca2+ with PLDbetacat (PLD with a deleted regulatory C2 domain) increases the affinity of the protein for the activator phosphatidylinositol 4,5-bisphosphate. Ca2+ binding to the C2 domain stimulates phosphatidylcholine binding but inhibits phosphatidylinositol 4,5-bisphosphate | Arabidopsis thaliana |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild-type PLDbeta, PLDbetacat with a deleted C2 domain, expression in Escherichia coli | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELTA1-158 | PLD with a deleted regulatory C2 domain exhibits Ca2+-dependent activity, is much less active and requires a higher level of Ca2+ than the full-length enzyme PLDbeta | Arabidopsis thaliana |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | interacts with the catalytic regions of the enzyme. PLDbetacat (PLD with a deleted regulatory C2 domain) exhibits Ca2+-dependent activity, is much less active and requires a higher level of Ca2+ than the full-length enzyme PLDbeta. The interaction of Ca2+ with PLDbetacat (PLD with a deleted regulatory C2 domain) increases the affinity of the protein for the activator phosphatidylinositol 4,5-bisphosphate. Ca2+ binding to the C2 domain stimulates phosphatidylcholine binding but inhibits phosphatidylinositol 4,5-bisphosphate | Arabidopsis thaliana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | - |
Arabidopsis thaliana | 1,2-diacylglycerophosphate + choline | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phospholipase Dbeta | - |
Arabidopsis thaliana |
| PLDbeta | - |
Arabidopsis thaliana |
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