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Literature summary for 3.1.4.4 extracted from

  • Virto, C.; Svensson, I.; Adlercreutz, P.
    Hydrolytic and transphosphatidylation activities of phospholipase D from Savoy cabbage towards lysophosphatidylcholine (2000), Chem. Phys. Lipids, 106, 41-51.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CTAB inhibitory at concentrations at or above that of 1 lysophosphatidylcholine Brassica oleracea var. capitata
SDS inhibitory at concentrations at or above that of 1 lysophosphatidylcholine Brassica oleracea var. capitata
Triton X-100 inhibitory at concentrations at or above that of 1 lysophosphatidylcholine Brassica oleracea var. capitata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
lysophosphatidylcholine at 4 mM Ca2+ Brassica oleracea var. capitata
3
-
lysophosphatidylcholine at 40 mM Ca2+ Brassica oleracea var. capitata
388
-
glycerol transphosphatidylation Brassica oleracea var. capitata

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ absolute requirement, optimal at 40 mM Brassica oleracea var. capitata

Organic Solvent Stability

Organic Solvent Comment Organism
Glycerol for measurement of transfer reaction, 2 M included in media to suppress hydrolytic reaction Brassica oleracea var. capitata

Organism

Organism UniProt Comment Textmining
Brassica oleracea var. capitata
-
var. sabauda
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lysophosphatidylcholine + glycerol
-
Brassica oleracea var. capitata ?
-
?
lysophosphatidylcholine + H2O about 20 times slower than reaction of phosphatidylcholine Brassica oleracea var. capitata choline + lysophosphatidate
-
?
phosphatidylcholine + glycerol
-
Brassica oleracea var. capitata ?
-
?
phosphatidylcholine + H2O
-
Brassica oleracea var. capitata choline + phosphatidate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Brassica oleracea var. capitata