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Literature summary for 3.1.4.4 extracted from
- Marked activation of the N-acylphosphatidylethanolamine-hydrolyzing phosphodiesterase by divalent cations (2001), Biochim. Biophys. Acta, 1532, 121-127.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | up to 0.4%, inhibitory above | Rattus norvegicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 10 mM, complete inhibition | Rattus norvegicus | |
| Fe2+ | 10 mM, complete inhibition | Rattus norvegicus | |
| Hg2+ | 10 mM, complete inhibition | Rattus norvegicus | |
| Triton X-100 | activating up to 0.4%, inhibitory above | Rattus norvegicus | |
| Zn2+ | 10 mM, complete inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
N-palmitoyl-phosphatidylethanolamine | pH 7.5, 37°C, in presence of Cacl2 | Rattus norvegicus | |
| 0.045 | - |
N-palmitoyl-phosphatidylethanolamine | pH 7.5, 37°C, in presence of Triton X-100 | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| particle-bound | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | marked stimulation | Rattus norvegicus | |
| Ca2+ | 10 mM, up to 30-fold activation | Rattus norvegicus | |
| Co2+ | marked stimulation | Rattus norvegicus | |
| Mg2+ | marked stimulation | Rattus norvegicus | |
| Mn2+ | marked stimulation | Rattus norvegicus | |
| Ni2+ | marked stimulation | Rattus norvegicus | |
| Sr2+ | marked stimulation | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
pH 7.5, 37°C | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-arachidonoyl-phosphatidylethanolamine + H2O | - |
Rattus norvegicus | phosphatidic acid + N-arachidonoylethanolamine | - |
? | |
| N-palmitoyl-phosphatidylethanolamine + H2O | - |
Rattus norvegicus | phosphatidic acid + N-palmitoylethanolamine | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
10 min, complete loss of activity | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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