Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.4 extracted from

  • Novotna, Z.; Kas, J.; Daussant, J.; Sajdok, J.; Valentova, O.
    Purification and characterisation of rape seed phospholipase D (1999), Plant Physiol. Biochem., 37, 531-537.
No PubMed abstract available
Search for more literature for 3.1.4.4

Activating Compound

Activating Compound Comment Organism Structure
SDS
-
 Brassica napus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1 4 phosphatidylcholine depends on SDS Brassica napus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 20-100 mM required for maximal activity in plants Brassica napus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000 105000 gel filtration, SDS-PAGE Brassica napus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipid + H2O Brassica napus phosphoric ester hydrolysis phosphatidic acid + alcohol
-
 ?

Organism

Organism UniProt Comment Textmining
Brassica napus
-
 Winter rape
-

Purification (Commentary)

Purification (Comment) Organism
complete to homogeneity Brassica napus

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Brassica napus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + H2O
-
 Brassica napus 1,2-diacylglycerophosphate + choline
-
 ?
phosphatidylethanolamine + H2O
-
 Brassica napus 1,2-diacylglycerophosphate + ethanolamine
-
 ?
phospholipid + H2O phosphoric ester hydrolysis Brassica napus phosphatidic acid + alcohol
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
 Brassica napus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7
-
 Brassica napus