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Literature summary for 3.1.4.39 extracted from

  • van Meeteren, L.A.; Brinkmann, V.; Saulnier-Blache, J.S.; Lynch, K.R.; Moolenaar, W.H.
    Anticancer activity of FTY720: phosphorylated FTY720 inhibits autotaxin, a metastasis-enhancing and angiogenic lysophospholipase D (2008), Cancer Lett., 266, 203-208.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.39

Cloned(Commentary)

Cloned (Comment) Organism
human ATX cDNA cloned with a C-terminal His-tag in pcDNA3 for transfection of HEK293T cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-amino-2-(2-(4-octylphenyl) ethyl)propan-1,3-diol synonyms FTY720, finolimod, competitive inhibition Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lysophosphatidylcholine + H2O Homo sapiens
-
 lysophosphatidic acid + choline
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant ATX is purified using TALON resin beads Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lysophosphatidylcholine + H2O
-
 Homo sapiens lysophosphatidic acid + choline
-
 ?
lysophosphatidylcholine + H2O radiolabeled substrate and fluorogenic substrate FS-3 Homo sapiens lysophosphatidic acid + choline
-
 ?
p-nitrophenyl thymidine 5'-monophosphate + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
ATX
-
 Homo sapiens
autotaxin
-
 Homo sapiens
lysophospholipase D
-
 Homo sapiens
NPP2
-
 Homo sapiens
nucleotide phosphodiesterase/pyrophosphatase (NPP)
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0002
-
 2-amino-2-(2-(4-octylphenyl) ethyl)propan-1,3-diol substrate p-nitrophenyl thymidine 5-monophosphate, pH 8.0, 30 min of incubation at 37°C, measuring absorbance at 405 nm, twofold higher KI compared to inhibitor S1P Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0003
-
 to 0.0004, substrate p-nitrophenyl thymidine 5-monophosphate, pH 8.0, 30 min of incubation at 37°C, measuring absorbance at 405 nm, compared to inhibitor S1P 0.0001 mM Homo sapiens 2-amino-2-(2-(4-octylphenyl) ethyl)propan-1,3-diol